神经母细胞瘤与胶质瘤杂交细胞中乙酰胆碱释放的调节
Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells.
作者信息
McGee R, Simpson P, Christian C, Mata M, Nelson P, Nirenberg M
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1314-8. doi: 10.1073/pnas.75.3.1314.
Abstract
Neuroblastoma x glioma NG108-15 hybrid cells exposed to N6, O2'-dibutyryladenosine 3':5'-cyclic monophosphate for several days release [3H]acetylcholine in response to serotonin, prostaglandin F2alpha, KCl, or veratridine. NG108-15 cells grown in the absence of dibutyrul cyclic AMP do not respond to an excitatory stimulus by releasing [3H]acetylcholine but can be shifted to a responsive state by treatment with dibutyryl cyclic AMP. Thus, the reactions that are required for acetylcholine release can be regulated in NG108-15 cells, thereby regulating the ability of cells to form synapses and the efficiency of synaptic communication.
摘要
暴露于N6, O2'-二丁酰腺苷3':5'-环一磷酸数天的神经母细胞瘤x胶质瘤NG108-15杂交细胞，会对血清素、前列腺素F2α、氯化钾或藜芦碱作出反应而释放[3H]乙酰胆碱。在没有二丁酰环磷腺苷的情况下生长的NG108-15细胞，不会通过释放[3H]乙酰胆碱对兴奋性刺激作出反应，但可以通过用二丁酰环磷腺苷处理而转变为反应状态。因此，NG108-15细胞中乙酰胆碱释放所需的反应可以被调节，从而调节细胞形成突触的能力和突触通讯的效率。
相似文献
1
Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells.神经母细胞瘤与胶质瘤杂交细胞中乙酰胆碱释放的调节
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1314-8. doi: 10.1073/pnas.75.3.1314.
2
Choline uptake by the neuroblastoma x glioma hybrid, NG108-15.神经母细胞瘤与胶质瘤杂交细胞NG108-15对胆碱的摄取。
J Neurochem. 1980 Oct;35(4):829-37. doi: 10.1111/j.1471-4159.1980.tb07079.x.
3
A new capillary tube system for measuring the uptake and release of materials from cultured cells.
Anal Biochem. 1980 Jan 15;101(2):320-6. doi: 10.1016/0003-2697(80)90194-3.
4
5
Overexpression of adhesion molecule L1 in NG108-15 neuroblastoma X glioma hybrid cells enhances dibutyryl cyclic AMP-induced neurite outgrowth and functional synapse formation with myotubes.黏附分子L1在NG108 - 15神经母细胞瘤X胶质瘤杂交细胞中的过表达增强了二丁酰环磷酸腺苷诱导的神经突生长以及与肌管的功能性突触形成。
J Neurochem. 1997 Jun;68(6):2291-9. doi: 10.1046/j.1471-4159.1997.68062291.x.
6
Induction of differentiation increases Met5-enkephalin and Leu5-enkephalin content in NG108-15 hybrid cells: an immunocytochemical and biochemical analysis.诱导分化增加NG108 - 15杂交细胞中蛋氨酸脑啡肽和亮氨酸脑啡肽的含量：免疫细胞化学和生化分析
J Neurosci. 1983 Sep;3(9):1713-27. doi: 10.1523/JNEUROSCI.03-09-01713.1983.
7
Pharmacologic responses of cells of a neuroblastoma X glioma hybrid clone and modulation of synapses between hybrid cells and mouse myotubes.神经母细胞瘤X胶质瘤杂交克隆细胞的药理反应及杂交细胞与小鼠肌管之间突触的调节
Brain Res. 1978 May 26;147(2):261-76. doi: 10.1016/0006-8993(78)90839-9.
8
Enhanced acetylcholine secretion in neuroblastoma x glioma hybrid NG108-15 cells transfected with rat choline acetyltransferase cDNA.用大鼠胆碱乙酰转移酶cDNA转染的神经母细胞瘤x胶质瘤杂交NG108-15细胞中乙酰胆碱分泌增强。
FEBS Lett. 1992 Dec 21;314(3):409-12. doi: 10.1016/0014-5793(92)81516-o.
9
On the relationship between (3H)choline uptake activation and (3H)acetylcholine release.
J Neurochem. 1977 Oct;29(4):681-7. doi: 10.1111/j.1471-4159.1977.tb07786.x.
10
Acetylcholine accumulation and release by hybrid NG108-15, glioma and neuroblastoma cells--role of a 16kDa membrane protein in release.
Neurochem Int. 1994 Aug;25(2):103-9. doi: 10.1016/0197-0186(94)90029-9.
引用本文的文献
1
Mouse Neuroblastoma CB Cannabinoid Receptor-Stimulated [S]GTPɣS Binding: Total and Antibody-Targeted Gα Protein-Specific Scintillation Proximity Assays.小鼠神经母细胞瘤CB大麻素受体刺激的[S]GTPγS结合：总结合及抗体靶向的Gα蛋白特异性闪烁邻近分析
Methods Enzymol. 2017;593:1-21. doi: 10.1016/bs.mie.2017.06.028. Epub 2017 Jul 19.
2
Botulinum toxin a inhibits acetylcholine release from cultured neurons in vitro.肉毒杆菌毒素 A 抑制体外培养神经元中乙酰胆碱的释放。
In Vitro Cell Dev Biol Anim. 1993 Jun;29(6):456-60. doi: 10.1007/BF02639379.
3
Neurotransmitter release: vacuolar ATPase V0 sector c-subunits in possible gene or cell therapies for Parkinson's, Alzheimer's, and psychiatric diseases.神经递质释放：液泡型ATP酶V0扇区c亚基在帕金森病、阿尔茨海默病和精神疾病的可能基因或细胞治疗中的作用
J Physiol Sci. 2017 Jan;67(1):11-17. doi: 10.1007/s12576-016-0462-3. Epub 2016 Jun 11.
4
Development of a Highly Sensitive Cell-Based Assay for Detecting Botulinum Neurotoxin Type A through Neural Culture Media Optimization.通过神经培养基优化开发一种用于检测A型肉毒杆菌神经毒素的高灵敏度细胞检测方法。
J Biomol Screen. 2016 Jan;21(1):65-73. doi: 10.1177/1087057115608103. Epub 2015 Sep 29.
5
Voltage-gated sodium channel expression and action potential generation in differentiated NG108-15 cells.电压门控钠离子通道在分化的 NG108-15 细胞中的表达和动作电位产生。
BMC Neurosci. 2012 Oct 25;13:129. doi: 10.1186/1471-2202-13-129.
6
Model for studying Clostridium botulinum neurotoxin using differentiated motor neuron-like NG108-15 cells.利用分化的神经元样 NG108-15 细胞研究肉毒梭菌神经毒素的模型。
Biochem Biophys Res Commun. 2012 Oct 19;427(2):426-30. doi: 10.1016/j.bbrc.2012.09.082. Epub 2012 Sep 20.
7
Ect2, an ortholog of Drosophila Pebble, regulates formation of growth cones in primary cortical neurons.Ect2，果蝇 Pebble 的同源物，调节初级皮质神经元生长锥的形成。
Neurochem Int. 2012 Nov;61(6):854-8. doi: 10.1016/j.neuint.2012.02.011. Epub 2012 Feb 15.
8
Role of key aromatic residues in the ligand-binding domain of alpha7 nicotinic receptors in the agonist action of beta-amyloid.关键芳香残基在配体结合域α7 烟碱型乙酰胆碱受体在β-淀粉样肽激动剂作用中的作用。
J Biol Chem. 2011 Sep 30;286(39):34373-81. doi: 10.1074/jbc.M111.241299. Epub 2011 Aug 2.
9
Ect2, an ortholog of Drosophila's pebble, negatively regulates neurite outgrowth in neuroblastoma × glioma hybrid NG108-15 cells.Ect2，果蝇 pebble 的同源物，负调控神经母细胞瘤-胶质细胞瘤杂交细胞系 NG108-15 中的神经突生长。
Cell Mol Neurobiol. 2011 Jul;31(5):663-8. doi: 10.1007/s10571-011-9668-3. Epub 2011 Feb 25.
10
Mutant SOD1 impairs axonal transport of choline acetyltransferase and acetylcholine release by sequestering KAP3.突变型超氧化物歧化酶1通过隔离KAP3损害胆碱乙酰转移酶的轴突运输和乙酰胆碱释放。
Hum Mol Genet. 2009 Mar 1;18(5):942-55. doi: 10.1093/hmg/ddn422. Epub 2008 Dec 16.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Electrophoresis of acetylcholine, choline and related compounds.乙酰胆碱、胆碱及相关化合物的电泳
Biochem Pharmacol. 1967 Jul 7;16(7):1386-8. doi: 10.1016/0006-2952(67)90174-8.
3
Brain acetylcholine studies: a new extraction procedure.
J Neurochem. 1966 Dec;13(12):1533-44. doi: 10.1111/j.1471-4159.1966.tb04318.x.
4
High affinity transport of choline into synaptosomes of rat brain.胆碱向大鼠脑突触体的高亲和力转运。
J Neurochem. 1973 Dec;21(6):1355-74. doi: 10.1111/j.1471-4159.1973.tb06022.x.
5
The determination of picomole amounts of acetylcholine in mammalian brain.哺乳动物脑中皮摩尔量乙酰胆碱的测定。
J Neurochem. 1973 Jan;20(1):1-8. doi: 10.1111/j.1471-4159.1973.tb12097.x.
6
Morphine antagonizes the action of prostaglandin in neuroblastoma cells but not of prostaglandin and noradrenaline in glioma and glioma times fibroblast hybrid cells.吗啡可拮抗神经母细胞瘤细胞中前列腺素的作用，但对胶质瘤细胞以及胶质瘤与成纤维细胞杂交细胞中的前列腺素和去甲肾上腺素则无此作用。
FEBS Lett. 1974 Dec 15;49(2):260-3. doi: 10.1016/0014-5793(74)80525-9.
7
The ultrastructure of neuroblastoma glioma somatic cell hybrids. Expression of neuronal characteristics stimulated by dibutyryl adenosine 3',5' cyclic monophosphate.神经母细胞瘤-胶质瘤体细胞杂种的超微结构。二丁酰腺苷3'，5'-环磷酸刺激神经元特征的表达。
J Cell Biol. 1974 Nov;63(2 Pt 1):691-9. doi: 10.1083/jcb.63.2.691.
8
9
Muscarinic response to acetylcholine in neuroblastoma times glioma hybrid cells.
Nature. 1975 Jun 12;255(5509):558-60. doi: 10.1038/255558a0.
10
Dopamine depolarising response in a vertebrate neuronal somatic cell hybrid.
Nature. 1975 May 15;255(5505):235-6. doi: 10.1038/255235a0.
Zotero 插件