Oroszlan S, Henderson L E, Stephenson J R, Copeland T D, Long C W, Ihle J N, Gilden R V
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1404-8. doi: 10.1073/pnas.75.3.1404.
The amino- and carboxyl-terminal amino acid sequences of proteins (p10, p12, p15, and p30) coded by the gag gene of Rauscher and AKR murine leukemia viruses were determined. Among these proteins, p15 from both viruses appears to have a blocked amino end. Proline was found to be the common NH(2) terminus of both p30s and both p12s, and alanine of both p10s. The amino-terminal sequences of p30s are identical, as are those of p10s, while the p12 sequences are clearly distinctive but also show substantial homology. The carboxyl-terminal amino acids of both viral p30s and p12s are leucine and phenylalanine, respectively. Rauscher leukemia virus p15 has tyrosine as the carboxyl terminus while AKR virus p15 has phenylalanine in this position. The compositional and sequence data provide definite chemical criteria for the identification of analogous gag gene products and for the comparison of viral proteins isolated in different laboratories. On the basis of amino acid sequences and the previously proposed H-p15-p12-p30-p10-COOH peptide sequence in the precursor polyprotein, a model for cleavage sites involved in the post-translational processing of the precursor coded for by the gag gene is proposed.
确定了劳舍尔和AKR鼠白血病病毒gag基因编码的蛋白质（p10、p12、p15和p30）的氨基末端和羧基末端氨基酸序列。在这些蛋白质中，两种病毒的p15似乎都有一个封闭的氨基末端。发现脯氨酸是两种p30和两种p12的共同氨基末端，丙氨酸是两种p10的共同氨基末端。p30的氨基末端序列相同，p10的也相同，而p12的序列明显不同，但也显示出高度同源性。两种病毒的p30和p12的羧基末端氨基酸分别是亮氨酸和苯丙氨酸。劳舍尔白血病病毒p15的羧基末端是酪氨酸，而AKR病毒p15在这个位置是苯丙氨酸。组成和序列数据为鉴定类似的gag基因产物以及比较在不同实验室分离的病毒蛋白提供了明确的化学标准。基于氨基酸序列以及先前提出的前体多蛋白中的H-p15-p12-p30-p10-COOH肽序列，提出了一个gag基因编码的前体翻译后加工中涉及的切割位点模型。
