Wang T, Fu F H, Han B, Zhang L M, Zhang X M
Department of Pharmacology, Shandong University School of Medicine, Wenhua Xi Road, Jinan, Shandong, PR China.
Exp Clin Endocrinol Diabetes. 2011 Jan;119(1):36-40. doi: 10.1055/s-0030-1261933. Epub 2010 Aug 5.
Increasing studies have shown that the patients with diabetes mellitus have an increased risk of cognitive impairment, dementia, and neurodegeneration. The present study was designed to evaluate the effect of aspirin on diabetes-associated learning and memory decline in mice. Diabetes was induced by a single intraperitoneal injection of streptozocin (150 mg/kg body weight) in C57BL/6 mice. The mice were administered with aspirin at a dose of 30 mg/kg by intragastric administration once a day for 1, 4 or 8 weeks respectively. 8 weeks after aspirin or vehicle treatment, the effect of aspirin on diabetes-associated learning and memory decline in mice was investigated by evaluating the mean escape latency and the percentage of time spent in target quadrant. The TNF-α, IL-1β contents, and acetylcholinesterase activity in hippocampus were assayed as well. The results showed that administration with aspirin for 4 weeks or 8 weeks significantly reduced the mean escape latency, the acetylcholinesterase activity, the TNF-α, IL-1β levels and increased the percentage of time spent in target quadrant. However, treatment with aspirin for 1 week did not ameliorate diabetes-associated learning and memory decline. The present study demonstrated that long-term aspirin treatment attenuates diabetes-associated learning and memory decline in mice, and that the effect of aspirin was related to its anti-inflammatory potency.
越来越多的研究表明，糖尿病患者发生认知障碍、痴呆和神经退行性变的风险增加。本研究旨在评估阿司匹林对小鼠糖尿病相关学习和记忆衰退的影响。通过对C57BL/6小鼠单次腹腔注射链脲佐菌素（150 mg/kg体重）诱导糖尿病。分别以30 mg/kg的剂量每天一次对小鼠进行灌胃给予阿司匹林，持续1、4或8周。在阿司匹林或赋形剂治疗8周后，通过评估平均逃避潜伏期和在目标象限花费的时间百分比，研究阿司匹林对小鼠糖尿病相关学习和记忆衰退的影响。同时检测海马中的TNF-α、IL-1β含量以及乙酰胆碱酯酶活性。结果显示，给予阿司匹林4周或8周可显著降低平均逃避潜伏期、乙酰胆碱酯酶活性、TNF-α、IL-1β水平，并增加在目标象限花费的时间百分比。然而，给予阿司匹林1周并未改善糖尿病相关的学习和记忆衰退。本研究表明，长期使用阿司匹林可减轻小鼠糖尿病相关的学习和记忆衰退，且阿司匹林的作用与其抗炎效力有关。
