Child Dental Health, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK.
J Biomed Mater Res B Appl Biomater. 2010 Oct;95(1):62-8. doi: 10.1002/jbm.b.31683.
Treatment of early root caries using resin adhesives to stabilize demineralized dentine and deprive bacteria of nutrients is a recognized and conservative treatment option. Essential to its success is a stable resin-dentine interface, with effective infiltration of demineralized dentine and adequate resin curing. The objective of this study was therefore to examine degree of cure (DoC) and depth of penetration of dentine adhesives in demineralized dentine using micro-Raman spectroscopy. Three commercially available adhesives were applied essentially according to manufacturers' instructions to visibly wet demineralized dentine, with two coats of primer to reduce dilution. Specimens were sectioned perpendicular to the bonded interface and micro-Raman spectroscopy performed on the cross section. Molecules associated with the adhesive were detected to depths greater than 100 μm indicating a thicker hybrid layer than seen in sound dentine. The hybrid layer showed an increase in proportion of aliphatic C=C double bonds at the base of the hybrid layer, which may be a consequence of reduced DoC or phase separation. Micro-Raman spectra suggest deep zones contain an increased concentration of HEMA and a lower concentration of BisGMA, which is likely to be less stable and may contribute to early breakdown of the dentine adhesive interface.
使用树脂黏合剂稳定脱矿牙本质并使细菌失去营养来源来治疗早期根面龋是一种公认的保守治疗方法。成功的关键是稳定的树脂-牙本质界面，实现脱矿牙本质的有效渗透和足够的树脂固化。因此，本研究旨在使用微拉曼光谱检查脱矿牙本质中牙本质黏合剂的固化程度（DoC）和渗透深度。根据制造商的说明，将三种市售黏合剂基本应用于明显湿润的脱矿牙本质，使用两层底漆以减少稀释。将样本垂直于粘结界面进行切片，并在横截面上进行微拉曼光谱分析。检测到与黏合剂相关的分子渗透深度超过 100μm，表明形成的混合层比正常牙本质更厚。混合层底部的脂肪族 C=C 双键比例增加，这可能是由于 DoC 降低或相分离所致。微拉曼光谱表明深层区域含有更高浓度的 HEMA 和更低浓度的 BisGMA，这可能不太稳定，并且可能导致牙本质黏合剂界面的早期失效。
