Department of Dental Materials, School of Dentistry, Campus de Cartuja s/n, University of Granada, Granada, Spain.
J Dent. 2012 Sep;40(9):756-65. doi: 10.1016/j.jdent.2012.05.007. Epub 2012 Jun 1.
The objective of the study was to determine the efficacy of a ZnO-doped etch and rinse adhesive in decreasing MMPs-mediated collagen degradation at the resin-dentine hybrid layer, and increasing bonding stability.
C-terminal telopeptide concentrations (ICTP) were determined after 24h, 1wk and 4wk in human dentine beams. Dentine was treated: (1) 37% phosphoric acid for 15s (PA), (2) PA-etched dentine infiltrated with Single Bond (SB), (3) PA-etched dentine infiltrated with ZnO doped SB (ZnO particles--10wt%--were added to the bonding resin) (ZnO-SB), and (4) Clearfil SE Bond primed-dentine was infiltrated with Clearfil SE bonding resin (CSE). Microtensile bond strength (MTBS) was assessed for the different groups at 24h and after 3months. Debonded dentine surfaces were studied by scanning electron microscopy.
MMPs-mediated collagen degradation occurred in demineralized dentine (PA). Resin infiltration decreased collagen degradation. The lowest collagen degradation was found for Zn-doped SB, followed by CSE. When these adhesives were applied, ICTP values did not change throughout the study period. At 24h, similar MTBS was attained for all adhesives. Only SB decreased MTBS after three months.
Addition of ZnO particles to SB produced a reduction in dentine collagen degradation and increased resin-dentine bonds durability. In Zn-doped adhesive interfaces, a calcium phosphate layer and tubular occlusion was encountered at the debonded interface.
ZnO particles addition into the bonding resin of SB makes a breakthrough to prevent the hybrid layer degradation and to preserve its bonding efficacy overtime.
本研究旨在评估掺锌纳米氧化锌酸蚀剂对减少 MMPs 介导的牙本质胶原降解、提高粘结稳定性的效果。
采用人牙本质梁模型，通过检测 C 末端肽（ICTP）的浓度，分别于 24h、1 周和 4 周观察 37%磷酸酸蚀 15s（PA）、PA 酸蚀后用单组分粘结剂（SB）处理、PA 酸蚀后用掺锌纳米氧化锌 SB 处理（粘结剂中加入 10wt%的 ZnO 纳米颗粒）（ZnO-SB）和 PA 酸蚀后用 Clearfil SE Bond 预处理牙本质（CSE）组牙本质胶原的降解情况。分别于即刻和 3 个月后测试微拉伸粘结强度（MTBS）。用扫描电子显微镜观察粘结界面脱粘的牙本质表面。
脱矿牙本质（PA）中 MMPs 介导的胶原降解。树脂渗透可减少胶原降解。掺锌 SB 处理后胶原降解最少，其次是 CSE。当使用这些粘结剂时，ICT 浓度在整个研究期间均未发生变化。24h 时，所有粘结剂的 MTBS 相似。仅 SB 在 3 个月后降低了 MTBS。
在 SB 粘结剂中添加 ZnO 纳米颗粒可减少牙本质胶原降解，提高树脂-牙本质粘结的耐久性。在掺锌粘结剂界面，在脱粘界面上观察到磷酸钙层和管腔闭塞。
在 SB 粘结剂中添加 ZnO 纳米颗粒可防止混合层降解，从而提高粘结效果的耐久性。
