Department of Radiology, University of Giessen, Germany.
Atherosclerosis. 2010 Oct;212(2):444-50. doi: 10.1016/j.atherosclerosis.2010.07.010. Epub 2010 Jul 17.
To investigate the distribution of vasa vasorum (VV) relative to advanced atherosclerotic lesions (calcified, fibrotic or hemorrhaged) along the aortic wall of apoE-/-/LDL-/- mice at the age of 25 and 80 weeks using high-resolution nano-CT.
Aortas from male apoE-/-/LDL-/- mice at the age of 25 weeks (n=4) and 80 weeks (n=7) were infused in situ with contrast agent and harvested for scanning with nano-CT. The spatial distribution of vasa vasorum [number and area/cross-section (mm2)] was compared to aortic luminal cross-sectional area and plaque cross-sectional area in the ascending aorta, aortic arch and descending aorta. Results were complemented with co-localized histology.
The number and total luminal cross-sectional area of VV showed a significant decrease in the ascending aorta and aortic arch from 25 to 80 weeks but not in the descending aorta. The number and cross-sectional area of VV showed significant local differences depending on whether it was near a fibrotic, and hemorrhaged or calcified plaque in animals at the age of 80 weeks. Area of VV progressively increased along the aorta from least in the ascending aorta<aortic arch<descending aorta in animals at the age of 80 weeks and is inverse in animals aged 25 weeks.
Atherosclerotic lesion type is correlated to the number and cross-sectional area of VV in different aortic segments in apoE-/-/LDL-/- mice. The chronological development of VV along the aorta proceeds distally from the ascending aorta and aortic arch.
使用高分辨率纳米 CT 研究 apoE-/-/LDL-/- 小鼠 25 周和 80 周时主动脉壁中 vasa vasorum(VV)相对于晚期动脉粥样硬化病变(钙化、纤维化或出血)的分布。
对 25 周龄(n=4)和 80 周龄(n=7)雄性 apoE-/-/LDL-/- 小鼠的主动脉进行原位对比剂灌注,并进行纳米 CT 扫描。将 vasa vasorum 的空间分布[数量和面积/横截面(mm2)]与升主动脉、主动脉弓和降主动脉的管腔横截面面积和斑块横截面面积进行比较。结果辅以共定位组织学。
从 25 周到 80 周,升主动脉和主动脉弓的 VV 数量和总管腔横截面面积显著减少,但降主动脉没有。80 周龄动物中,VV 的数量和横截面积根据其是否靠近纤维化、出血或钙化斑块而存在显著的局部差异。80 周龄动物中,VV 的面积沿主动脉从升主动脉<主动脉弓<降主动脉逐渐增加,而 25 周龄动物则相反。
在 apoE-/-/LDL-/- 小鼠的不同主动脉节段中,动脉粥样硬化病变类型与 VV 的数量和横截面积相关。VV 沿主动脉的时间发展从升主动脉和主动脉弓向远端进行。