自发性早产妇女中线粒体孕激素受体多态性缺失。
Absence of mitochondrial progesterone receptor polymorphisms in women with spontaneous preterm birth.
机构信息
Eunice Kennedy Shriver NICHD MFMU Network, Bethesda, MD, USA.
出版信息
Reprod Sci. 2010 Oct;17(10):913-6. doi: 10.1177/1933719110374365. Epub 2010 Aug 6.
OBJECTIVE
The truncated mitochondrial progesterone receptor (PR-M) is homologous to nuclear PRs with the exception of an amino terminus hydrophobic membrane localization sequence, which localizes PR-M to mitochondria. Given the matrilineal inheritance of both spontaneous preterm birth (SPTB) and the mitochondrial genome, we hypothesized that (a) PR-M is polymorphic and (b) PR-M localization sequence polymorphisms could result in variable progesterone-mitochondrial effects and variable responsiveness to progesterone prophylaxis.
METHODS
Secondary analysis of DNA from women enrolled in a multicenter, prospective, study of 17 alpha-hydroxyprogesterone caproate (17OHPC) versus placebo for the prevention of recurrent SPTB. DNA was extracted from stored saliva.
RESULTS
The PR-M localization sequence was sequenced on 344 patients. Sequences were compared with the previously published 48 base-pair sequence, and all were identical.
CONCLUSIONS
We did not detect genetic variation in the mitochondrial localization sequence of the truncated PR-M in a group of women at high risk for SPTB.
目的
截断的线粒体孕激素受体（PR-M）与核 PR 具有同源性，除了一个氨基末端疏水性膜定位序列外，该序列将 PR-M 定位在线粒体中。鉴于自发性早产（SPTB）和线粒体基因组的母系遗传，我们假设（a）PR-M 是多态性的，（b）PR-M 定位序列多态性可能导致孕激素-线粒体作用的可变性和对孕激素预防的反应性的可变性。
方法
对参加多中心前瞻性研究的妇女的 DNA 进行二次分析，该研究比较了 17α-羟孕酮己酸酯（17OHPC）与安慰剂预防复发性 SPTB 的效果。从储存的唾液中提取 DNA。
结果
对 344 名患者的 PR-M 定位序列进行了测序。将序列与先前发表的 48 个碱基序列进行比较，所有序列均相同。
结论
我们在一组有发生 SPTB 高风险的妇女中未发现截断的 PR-M 线粒体定位序列的遗传变异。
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