Morgan Thomas, Bahtiyar Mert O, Snegovskikh Victoria V, Schatz Frederick, Kuczynski Edward, Funai Edmund F, Dulay Antonette T, Huang Se-Te Joseph, Buhimschi Catalin S, Buhimschi Irina A, Fortunato Stephen J, Menon Ramkumar, Lockwood Charles J, Norwitz Errol R
Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut, USA.
Reprod Sci. 2008 Feb;15(2):147-55. doi: 10.1177/1933719107310990.
Progesterone supplementation can prevent preterm birth in some high-risk women. Progesterone binds to progesterone receptor (PR) and modulates the expression of target genes. This study investigates the association between single nucleotide polymorphisms (SNPs) in the PR gene and spontaneous preterm birth. DNA was extracted from consecutive patients with preterm birth (n = 78) and term controls (n = 415), and genotyping was performed for 3 PR SNPs (+331[G>A], + 770[C>T], +660[G>T]) using Sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Data were analyzed by chi(2) test and logistic regression analysis. Multivariate analysis showed no association between maternal carriage of minor + 331T, +770T, and/or +660T alleles and preterm birth when controlled for maternal age, ethnicity, gravidity, parity, prior preterm birth, route of delivery, or neonatal outcome. Carriage of +770T and +660T (but not +331T) was associated with preterm birth in women with a body mass index <18.5 kg/m(2) (relative risk, 10.8; 95% confidence interval, 1.4-82.6; P = .02). Maternal carriage of minor alleles of +331(G>A), +770(C>T), and +660(G> T) SNPs in the PR gene is not associated with spontaneous preterm birth.
补充孕酮可预防部分高危女性早产。孕酮与孕酮受体（PR）结合并调节靶基因的表达。本研究调查PR基因单核苷酸多态性（SNP）与自发性早产之间的关联。从连续的早产患者（n = 78）和足月对照者（n = 415）中提取DNA，并使用Sequenom基质辅助激光解吸/电离飞行时间质谱法对3个PR SNP（+331[G>A]、+770[C>T]、+660[G>T]）进行基因分型。通过卡方检验和逻辑回归分析对数据进行分析。多因素分析显示，在控制产妇年龄、种族、妊娠次数、产次、既往早产史、分娩方式或新生儿结局后，产妇携带次要的+331T、+770T和/或+660T等位基因与早产无关联。在体重指数<18.5 kg/m²的女性中，携带+770T和+660T（而非+331T）与早产相关（相对风险为10.8；95%置信区间为1.4 - 82.6；P = 0.02）。PR基因中+331（G>A）、+770（C>T）和+660（G>T）SNP的次要等位基因的产妇携带情况与自发性早产无关。
