Department of Chemical Engineering, Case Western Reserve University, Cleveland, Ohio 44106, USA.
J Biomed Mater Res A. 2010 Sep 15;94(4):1050-60. doi: 10.1002/jbm.a.32774.
Collagen-chondroitin sulfate biomaterial scaffolds have been used in a number of tissue-engineered products under development or in the clinics. In this article, we describe a new approach based on centrifugation for obtaining highly concentrated yet porous collagen scaffolds. Water uptake, chondroitin sulfate retention, morphology, mechanical properties, and tissue-engineering potential of the concentrated scaffolds were investigated. Our results show that the new approach can lead to scaffolds containing four times as much collagen as that in conventional unconcentrated scaffolds. Further, water uptake in the concentrated scaffolds was significantly greater while chondroitin sulfate retention in the concentrated scaffolds was unaffected. The value of mean pore diameter in the concentrated scaffolds was smaller than that in the unconcentrated scaffolds and the walls of the pores in the former comprised of a continuous sheet of collagen. The mechanical properties measured as moduli of elasticity in compression and tension were improved by as much as 30 times in the concentrated scaffolds. In addition, our tissue culture results with human mesenchymal stem cells and foreskin keratinocytes show that the new scaffolds can be used for cartilage and skin tissue-engineering applications.
胶原-硫酸软骨素生物材料支架已被用于许多正在开发或已在临床上应用的组织工程产品中。在本文中，我们描述了一种基于离心的新方法，用于获得高浓度但多孔的胶原支架。我们研究了浓缩支架的水摄取、硫酸软骨素保留、形态、机械性能和组织工程潜力。我们的结果表明，这种新方法可以使支架中的胶原含量是传统未浓缩支架中的四倍。此外，浓缩支架的水摄取量显著增加，而浓缩支架中的硫酸软骨素保留量不受影响。浓缩支架中平均孔径的值小于未浓缩支架中的值，并且前者的孔壁由连续的胶原片组成。在压缩和拉伸弹性模量方面测量的机械性能在浓缩支架中提高了多达 30 倍。此外，我们用人间质干细胞和成纤维细胞角质形成细胞的组织培养结果表明，新支架可用于软骨和皮肤组织工程应用。
