Wang Dong, Yang Zhongxu, Zhang Jianjun
Department of Neurosurgery, Tianjin Fourth Central Hospital, Tianjin, 300140, P R China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 Jul;24(7):801-5.
Bone marrow mesenchymal stem cells (BMSCs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on BMSCs transplantation, proliferation, and differentiation. To investigate the effect of BMSCs transplantation and mild hypothermia on repair of rat spinal cord injury (SCI).
Forty-five female adult SD rats (weighing 200-250 g) were made the models of hemitransection SCI and divided randomly into 3 groups according to different treatments: group A (SCI group), group B (BMSCs transplantation group), and group C [BMSCs transplantation combined with mild hypothermia (33-35 degrees C) group]. At 1, 2, 4, 6, and 8 weeks after injury, the function of hind limb was evaluated with Basso Beattie and Bresnahan (BBB) score and inclined plane test. At 4 weeks after injury, histopathology and BrdU immunohistochemistry staining were performed. At 8 weeks after injury, horseradish peroxidase (HRP) retrograde nerve trace and transmission electron microscope (TEM) testing were performed to observe the regeneration of axon.
After 4 weeks, the function of hind limb obviously recovered in groups B and C, there were significant differences in BBB score between groups B, C and group A (P < 0.05), between group B and group C (P < 0.05). There was no significant difference (P > 0.05) in tilt angle among 3 groups after 1 and 2 weeks, and there were significant differences (P < 0.05) among 3 groups after 4 weeks. HE staining showed that significant cavity could be seen in group A, little in group B, and no cavity in group C. BrdU immunohistochemistry staining showed that the number of positive cells was 0, 90.54 +/- 6.23, and 121.22 +/- 7.54 in groups A, B, and C, respectively; showing significant differences (P < 0.01) among 3 groups. HRP retrograde neural tracing observation showed that the number of HRP positive nerve fibers was 10.35 +/- 1.72, 43.25 +/- 2.65, and 84.37 +/- 4.59 in groups A, B, and C, respectively, showing significant differences (P < 0.01) among 3 groups. TEM observation showed that a great amount of unmyelinated nerve fibers and myelinated nerve fibers were found in central transverse plane in group C.
The BMSCs transplantation play an important role in promotion of recovering the function of hind limb after SCI, and mild hypothermia has synergism effects.
骨髓间充质干细胞（BMSCs）在修复神经损伤中发挥重要作用，同时外界温度对BMSCs移植、增殖及分化有显著影响。探讨BMSCs移植及亚低温对大鼠脊髓损伤（SCI）修复的影响。
45只成年雌性SD大鼠（体重200 - 250 g）制成脊髓半横断损伤模型，根据不同处理随机分为3组：A组（SCI组）、B组（BMSCs移植组）和C组[BMSCs移植联合亚低温（33 - 35℃）组]。在损伤后1、2、4、6和8周，采用Basso Beattie和Bresnahan（BBB）评分及斜板试验评估后肢功能。在损伤后4周，进行组织病理学和BrdU免疫组化染色。在损伤后8周，进行辣根过氧化物酶（HRP）逆行神经追踪及透射电镜（TEM）检测以观察轴突再生情况。
4周后，B组和C组后肢功能明显恢复，B组、C组与A组之间BBB评分差异有统计学意义（P < 0.05），B组与C组之间差异有统计学意义（P < 0.05）。1周和2周后3组间倾斜角度差异无统计学意义（P > 0.05），4周后3组间差异有统计学意义（P < 0.05）。HE染色显示，A组可见明显空洞，B组较少，C组无空洞。BrdU免疫组化染色显示，A组、B组和C组阳性细胞数分别为0、90.54±6.23和121.22±7.54；3组间差异有统计学意义（P < 0.01）。HRP逆行神经追踪观察显示，A组、B组和C组HRP阳性神经纤维数分别为10.35±1.72、43.25±2.65和84.37±4.59，3组间差异有统计学意义（P < 0.01）。TEM观察显示，C组中央横切面可见大量无髓神经纤维和有髓神经纤维。
BMSCs移植对促进SCI后后肢功能恢复起重要作用，亚低温具有协同作用。
