Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, Qalioubeya, Egypt.
Eur J Clin Invest. 2010 Nov;40(11):984-93. doi: 10.1111/j.1365-2362.2010.02352.x.
Inflammation and oxidative stress are associated with cardiovascular diseases and underlying atherosclerosis. The high density lipoprotein (HDL)-associated paraoxonase-1 (PON1) enzyme is known to be involved in the protection of serum lipids from such oxidation. Nonetheless, the disturbances of lipid profile during nematode-infected model have not yet been studied. Therefore, we aimed to explore the effects of Nippostrongylus brasiliensis infection in male Wistar rats, a model of human gastrointestinal nematode infections, on hepatic PON1 synthesis and the levels of lipid parameters.
Nippostrongylus brasiliensis-infected rats fed standard and high-fat diets. Serum paraoxonase and arylesterase activities were measured on day 0, 2, 4, 7, and 14 post-infection (PI). Hepatic PONs and pro-inflammatory cytokines mRNA expression levels were evaluated in a standard diet-fed groups, and the disturbances in lipid profile as well as the levels of thiobarbituric acid reactive species (TBARS) and oxidized-LDL (Ox-LDL) were measured in high-fat diet-fed groups.
We found that N. brasiliensis-infected rats fed the standard diet show a significant reduction in serum PON1 activity and down-regulation of hepatic PON1 mRNA expression as well as up-regulation of hepatic IL-1β, IL-β receptor (R), TNF-α, and TNFR1 mRNA expressions in association with hepatic recruitments of Kupffer cells and neutrohils. In the presence of the high-fat diet, N. brasiliensis infection increases serum triglycerides, total cholesterol, LDL/VLDL, TBARS and Ox-LDL as well as decreases serum HDL coinciding with a maximum serum PON1 reduction.
Nippostrongylus brasiliensis infection can induce atherogenic lipid profile and reduce serum PON1 activity.
炎症和氧化应激与心血管疾病和潜在的动脉粥样硬化有关。高密度脂蛋白（HDL）相关的对氧磷酶-1（PON1）酶被认为参与保护血清脂质免受这种氧化。然而，尚未研究线虫感染模型中脂质谱的紊乱。因此，我们旨在探讨巴西旋毛虫感染对雄性 Wistar 大鼠（人类胃肠道线虫感染的模型）肝 PON1 合成和脂质参数水平的影响。
巴西旋毛虫感染的大鼠喂食标准和高脂肪饮食。在感染后 0、2、4、7 和 14 天（PI）测量血清对氧磷酶和芳基酯酶活性。在标准饮食喂养组评估肝 PONs 和促炎细胞因子 mRNA 表达水平，在高脂肪饮食喂养组测量脂质谱紊乱以及丙二醛（TBARS）和氧化型 LDL（Ox-LDL）水平。
我们发现，喂食标准饮食的巴西旋毛虫感染大鼠的血清 PON1 活性显著降低，肝 PON1 mRNA 表达下调，以及肝 IL-1β、IL-β 受体（R）、TNF-α 和 TNFR1 mRNA 表达上调，同时伴有肝库普弗细胞和中性粒细胞的募集。在高脂肪饮食的存在下，巴西旋毛虫感染增加了血清甘油三酯、总胆固醇、LDL/VLDL、TBARS 和 Ox-LDL，同时降低了血清 HDL，与血清 PON1 最大降低相一致。
巴西旋毛虫感染可诱导动脉粥样硬化脂质谱并降低血清 PON1 活性。
