Sci Sin. 1978 Jan-Feb;21(1):127-34.
An epithelioid cell line and a fusiform cell line were established from a tumor biopsy from a patient with nasopharyngeal carcinoma which as histologically diagnosed as a well differentiated squamous cell carcinoma. Based on studies of the cell growth pattern, chromosome analysis, heterotransplantation, and electron microscopy, these two cell lines were considered to be squamous carcinoma cells, and the fusiform cells might have originated from the epithelioid cells. There were many round cells on top of the epithelioid and fusiform cell sheets, many of which became continuously detached into the medium. Cultures initiated from these floating round cells grew into their original epithelioid or fusiform forms. No lymphoblastoid cell line could be established after cultivating these two cell lines for more than one year. No EB virus particle or early antigen could be detected in these two cell lines by means of electron microscopic examination and indirect immunofluorescence test.
从一名组织学诊断为高分化鳞状细胞癌的鼻咽癌患者的肿瘤活检中建立了一个上皮样细胞系和一个梭形细胞系。基于对细胞生长模式、染色体分析、异种移植和电子显微镜的研究，这两个细胞系被认为是鳞状癌细胞，梭形细胞可能起源于上皮样细胞。在上皮样和梭形细胞片层上方有许多圆形细胞，其中许多不断脱落到培养基中。从这些漂浮的圆形细胞开始培养，可长成其原始的上皮样或梭形形态。将这两个细胞系培养一年多后，未能建立起淋巴母细胞样细胞系。通过电子显微镜检查和间接免疫荧光试验，在这两个细胞系中均未检测到EB病毒颗粒或早期抗原。
