Yao K T
Hunan Medical University, Changsha.
Zhonghua Zhong Liu Za Zhi. 1990 Jan;12(1):34-6.
A novel epithelial cell line (designated as HNE-1), derived from nasopharyngeal carcinoma (NPC), was established and has passed more than 100 generations over one year. The NPC biopsy specimen was taken from a 27 year old man with poorly differentiated squamous cell carcinoma of the nasopharynx. The cultured cells showed polygonal shape and grew into multilayers under the inverted microscope. Electron microscopy showed that HNE-1 cells were characterized by bi-directional differentiation with some being poorly differentiated squamous carcinoma and the other poorly differentiated adenocarcinoma cells. A continuous positivity was showed by EBNA at subcultures 5-81. Tumorigenicity was demonstrated by heterotransplantation in BALB/c (nu/nu) mice, developing into well differentiated squamous carcinoma. Karyotype analysis showed aneuploidy with the modal chromosomal number 74-101 (5th-20th passages) and 15 marker chromosomes. The frequency of spontaneous sister chromatid exchange was very high in HNE-1 cells (87.6 +/- 0.4/cell).
建立了一种源自鼻咽癌（NPC）的新型上皮细胞系（命名为HNE-1），该细胞系在一年多的时间里已传代超过100次。NPC活检标本取自一名27岁患有鼻咽低分化鳞状细胞癌的男性。在倒置显微镜下，培养的细胞呈多边形，生长为多层。电子显微镜显示，HNE-1细胞的特征是双向分化，一些为低分化鳞状癌细胞，另一些为低分化腺癌细胞。在第5至81代传代培养时，EBNA持续呈阳性。通过在BALB/c（nu/nu）小鼠体内进行异种移植证明了其致瘤性，移植后发展为高分化鳞状癌。核型分析显示非整倍体，众数染色体数为74 - 101（第5至20代），并有15条标记染色体。HNE-1细胞中自发姐妹染色单体交换的频率非常高（87.6 +/- 0.4/细胞）。
