School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Bioresour Technol. 2010 Dec;101(24):9438-44. doi: 10.1016/j.biortech.2010.07.064. Epub 2010 Jul 18.
Autothermal thermophilic aerobic digestion (ATAD) is a promising process for sewage sludge stabilization. Batch experiments were conducted on sewage sludge collected from a municipal wastewater treatment plant in Shanghai, China, to evaluate the effectiveness of the ATAD system by determining changes in volatile suspended solids (VSSs) and to study its microbial diversity by denaturing gradient gel electrophoresis of 16S rRNA gene sequences amplified by PCR. The digestion system achieved rapid degradation of the organic substrate at 55 degrees C. The VSS was removed by up to 45.3% and 50.4% at 216 h and 264 h, respectively, while NH(4)(+)-N, chemical oxidation demand and total organic carbon of supernatant as well as total nitrogen did not exhibit obvious declines after 168 h. The microbial diversity changed during the thermophilic process as thermophiles belonging to the Hydrogenophilaceae, Thermotogaceae, Clostridiaceae and the genus Ureibacillus replaced less temperature-tolerant microorganisms such as Sphingobacteriaceae and the genus Trichococcus.
自热好氧高温消化（ATAD）是一种很有前途的污水污泥稳定化处理方法。采用来自中国上海某城市污水处理厂的污水污泥进行了批式实验，通过测定挥发性悬浮固体（VSS）的变化来评估 ATAD 系统的有效性，并通过聚合酶链式反应（PCR）扩增的 16S rRNA 基因序列的变性梯度凝胶电泳来研究其微生物多样性。在 55°C 时，消化系统实现了有机基质的快速降解。在 216 h 和 264 h 时，VSS 分别去除了 45.3%和 50.4%，而 NH(4)(+)-N、化学需氧量和上清液中的总有机碳以及总氮在 168 h 后没有明显下降。在高温过程中，微生物多样性发生了变化，属于噬氢菌科、热硫杆菌科、梭菌科和 Ureibacillus 属的嗜热菌取代了耐温性较低的微生物，如鞘脂单胞菌科和 Trichococcus 属。
