School of Environmental Science and Engineering, Shanghai Jiao Tong University, No. 800, Dongchuan Rd., Shanghai 200240, China.
Water Res. 2011 Nov 15;45(18):5959-68. doi: 10.1016/j.watres.2011.08.052. Epub 2011 Sep 3.
Two representative thermophilic bacterial strains (T1 and T2) were isolated from a one-stage autothermal thermophilic aerobic digestion pilot-scale reactor. 16S rRNA gene analysis indicated that they were Hydrogenophilaceae and Xanthomonodaceae. These isolated strains were inoculated separately and/or jointly in sewage sludge, to investigate their effects on sludge stabilization under thermophilic aerobic digestion condition. Four digestion conditions were tested for 480 h. Digestion without inoculation and inoculation with strain T2, as well as joint- inoculation with strains T1 and T2, achieved 32.6%, 43.0%, and 38.2% volatile solids (VS) removal, respectively. Removal in a digester inoculated with stain T1 only reached 27.2%. For the first 144 h, the three inoculated digesters all experienced higher VS removal than the digester without inoculations. Both specific thermophilic strains and micro-environment significantly affected the VS removal. DGGE profiles revealed that the isolated strains T1 and T2 can successfully establish in the thermophilic digesters. Other viable bacteria (including anaerobic or facultative microbes) also appeared in the digestion system, enhancing the microbial activity.
从一个一阶段自热嗜热好氧消化中试规模反应器中分离得到了两株具有代表性的嗜热细菌（T1 和 T2）。16S rRNA 基因分析表明，它们分别属于噬氢菌科和黄单胞菌科。将这些分离株分别和/或共同接种到污水污泥中，研究它们在嗜热好氧消化条件下对污泥稳定化的影响。测试了四种消化条件，持续 480 小时。未接种和接种 T2 菌株的消化，以及 T1 和 T2 菌株的联合接种，分别实现了 32.6%、43.0%和 38.2%的挥发性固体（VS）去除率。仅接种 T1 菌株的消化器的去除率仅达到 27.2%。在前 144 小时内，三个接种消化器的 VS 去除率均高于未接种的消化器。两种特定的嗜热菌株和微环境显著影响 VS 的去除率。DGGE 图谱显示，分离株 T1 和 T2 可以成功在嗜热消化器中建立。其他存活的细菌（包括厌氧或兼性微生物）也出现在消化系统中，增强了微生物的活性。
