砷抗性操纵子结构在铁氧化钩端螺旋菌和砷胁迫下的蛋白质组响应。

Arsenic resistance operon structure in Leptospirillum ferriphilum and proteomic response to arsenic stress.

机构信息

State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.

出版信息

Bioresour Technol. 2010 Dec;101(24):9811-4. doi: 10.1016/j.biortech.2010.07.043. Epub 2010 Jul 14.

DOI:10.1016/j.biortech.2010.07.043

PMID:20696570

Abstract

The response of Leptospirillum ferriphilum ML-04 to arsenic stress was analyzed using two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Thirty-eight of 65 significantly differentially expressed arsenic response proteins were identified, and 25 of them have known functions. Three proteins are arsenic resistance system (ARS) member proteins. Two ars operons appear to be present in this strain. In addition to the ARS system, phosphate regulation and glutathione (GSH) synthesis appear involved in As[V] and As[III] tolerance, respectively. These findings provide information potentially useful for the genetic engineering of arsenic resistant organisms.

摘要

采用二维电泳（2-DE）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析了铁氧化钩端螺旋菌 ML-04 对砷胁迫的响应。鉴定出 65 个差异表达的砷响应蛋白中有 38 个，其中 25 个具有已知功能。三种蛋白质是砷抗性系统（ARS）成员蛋白。该菌株似乎存在两个 ars 操纵子。除了 ARS 系统外，磷酸盐调节和谷胱甘肽（GSH）合成分别参与了 As[V]和 As[III]的耐受性。这些发现为砷抗性生物的基因工程提供了有价值的信息。