Held I R, Sayers S T, McLane J A
Neuroscience Research Laboratory, VA Hospital, Hines, IL 60141.
Alcohol. 1991 May-Jun;8(3):173-7. doi: 10.1016/0741-8329(91)90742-f.
The expression of the neuromuscular acetylcholine receptor (AChR) alpha-subunit gene was evaluated in soleus muscles from an animal model of chronic alcoholism. At 8 weeks of age, test rats were placed on a nutritionally complete liquid diet containing 6.7% ethanol (v/v). Age- and weight-matched control rats were pair-fed an isocaloric liquid diet. After a 16-week diet period, soleus muscles were obtained and total RNA and poly(A)+ RNA were isolated. Muscle RNA levels from ethanol-fed and control rats were comparable. AChR alpha-subunit mRNA was detected by hybridization of muscle poly(A)+ RNA with a 32P-labeled, complementary riboprobe. The steady-state level of AChR alpha-subunit mRNA was reduced by 39% (p less than 0.001) in soleus muscles from the ethanol-fed rats as compared to pair-fed controls. These results suggest that the expression of the AChR alpha-subunit gene is down-regulated after chronic ethanol exposure at a transcriptional or posttranscriptional level.
在慢性酒精中毒动物模型的比目鱼肌中评估神经肌肉乙酰胆碱受体（AChR）α亚基基因的表达。8周龄时，将试验大鼠置于含6.7%乙醇（体积/体积）的营养完全的液体饮食中。将年龄和体重匹配的对照大鼠配对喂食等热量的液体饮食。经过16周的饮食期后，获取比目鱼肌并分离总RNA和聚腺苷酸（poly(A)+）RNA。乙醇喂养大鼠和对照大鼠的肌肉RNA水平相当。通过肌肉聚腺苷酸（poly(A)+）RNA与32P标记的互补核糖探针杂交来检测AChRα亚基mRNA。与配对喂食的对照相比，乙醇喂养大鼠比目鱼肌中AChRα亚基mRNA的稳态水平降低了39%（p<0.001）。这些结果表明，慢性乙醇暴露后，AChRα亚基基因的表达在转录或转录后水平下调。
