Held I R
Neuroscience Research Laboratory, Veterans Administration Hospital, Hines, IL 60141.
Alcohol. 1992 Jan-Feb;9(1):79-82. doi: 10.1016/0741-8329(92)90014-2.
The effect of prolonged ethanol exposure on ribosomal RNA activity and the content of RNA and protein in skeletal muscles of 15- and 22-25-month-old rats was evaluated. Experimental rats were fed a liquid diet containing 6.7% ethanol for 2, 4 and 6 months, and control rats were pair-fed an isocaloric diet. The in vivo incorporation of [3H]puromycin into nascent peptides on messenger RNA-ribosome complexes was determined to assess muscle ribosomal RNA activity. This activity was significantly reduced in extensor digitorum longus and soleus muscles of rats fed ethanol for 2 months. While the total RNA content of these muscles was unchanged after feeding ethanol for 2, 4 and 6 months, their messenger RNA content was decreased from 26-34%. The total protein content was reduced after ethanol was consumed for 6 months. Taken together, the results suggest that alterations in the transcriptional or posttranscriptional control of messenger RNA may contribute toward the development of alcoholic myopathy after prolonged ethanol consumption.
评估了长期乙醇暴露对15月龄和22 - 25月龄大鼠骨骼肌核糖体RNA活性以及RNA和蛋白质含量的影响。将实验大鼠喂食含6.7%乙醇的液体饲料2、4和6个月，对照大鼠则喂食等热量的配对饲料。通过测定[3H]嘌呤霉素在信使核糖核酸 - 核糖体复合物上新生肽中的体内掺入量来评估肌肉核糖体RNA活性。喂食乙醇2个月的大鼠的趾长伸肌和比目鱼肌中的这种活性显著降低。虽然喂食乙醇2、4和6个月后这些肌肉的总RNA含量未变，但其信使核糖核酸含量下降了26 - 34%。摄入乙醇6个月后总蛋白质含量降低。综上所述，结果表明信使核糖核酸转录或转录后调控的改变可能在长期摄入乙醇后酒精性肌病的发展中起作用。
