Beckmann M P, Morrissey P J
Immunex Corporation, Seattle, Washington.
Curr Opin Immunol. 1991 Apr;3(2):247-51. doi: 10.1016/0952-7915(91)90060-e.
There have been several recent developments in the detection of cytokines, their receptors, and the genes that encode them. New methods for characterizing cytokines and lymphokines include the use of molecular hybridization techniques, bioassays and antibody-mediated detection systems. The most common techniques used for identifying and characterizing cytokine receptors rely upon the use of radiolabeled, fluoresceinated or biotinylated ligands.
近年来,在细胞因子、其受体以及编码这些受体的基因的检测方面有了一些新进展。用于表征细胞因子和淋巴因子的新方法包括分子杂交技术、生物测定法和抗体介导的检测系统。用于识别和表征细胞因子受体的最常用技术依赖于使用放射性标记、荧光标记或生物素化的配体。