Thorpe R, Wadhwa M, Bird C R, Mire-Sluis A R
Division of Immunobiology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK.
Blood Rev. 1992 Sep;6(3):133-48. doi: 10.1016/0268-960x(92)90025-l.
Accurate and sensitive methods for the measurement and detection of cytokines are an obvious pre-requisite for the study of cytokine biology, biochemistry and the possible involvement of these molecules in pathology. In this review, the various methods available for cytokine measurement and detection (bioassays, immunoassays and other procedures) are described and compared. A critical appraisal of the potential advantages and limitations of the techniques is included.
用于测量和检测细胞因子的准确且灵敏的方法,显然是研究细胞因子生物学、生物化学以及这些分子在病理学中可能参与情况的先决条件。在本综述中,描述并比较了可用于细胞因子测量和检测的各种方法(生物测定法、免疫测定法及其他方法)。还对这些技术的潜在优点和局限性进行了批判性评估。