Biomicrofluidics. 2010 Jun 29;4(2):022802. doi: 10.1063/1.3454129.
Progress in microelectrode-based technologies has facilitated the development of sophisticated methods for manipulating and separating cells, bacteria, and other bioparticles. For many of these various applications, the theoretical modeling of the electrical response of compartmentalized particles to an external field is important. In this paper we address the analysis of the interaction between cells immersed in rf fields. We use an integral formulation of the problem derived from a consideration of the charge densities induced at the interfaces of the particle compartments. The numerical solution by a boundary element technique allows characterization of their dielectric properties. Experimental validation of this theoretical model is obtained by investigating two effects: (1) The influence that dipolar "pearl chaining" has on the dielectrophoretic behavior of human T lymphocytes and (2) the frequency variation of the spin and orbital torques of approaching insulinoma beta-cells in a rotating field.
基于微电极技术的进展促进了用于操纵和分离细胞、细菌和其他生物颗粒的复杂方法的发展。对于这些各种应用中的许多应用，对分隔颗粒对外部场的电响应的理论建模很重要。在本文中，我们解决了浸入射频场中的细胞相互作用的分析问题。我们使用从颗粒隔室界面感应的电荷密度考虑得出的问题的积分公式。通过边界元技术的数值解允许对其介电特性进行表征。通过研究两个效应来验证该理论模型：（1）偶极“珍珠链”对人 T 淋巴细胞的介电泳行为的影响，以及（2）在旋转场中接近胰岛细胞瘤β细胞的自旋和轨道扭矩的频率变化。
