Université de Lyon - CNRS UMR5005 Laboratoire Ampère- Université Claude Bernard Lyon 1 - École Centrale de Lyon Ecully.
Biophys J. 2013 Apr 16;104(8):1805-11. doi: 10.1016/j.bpj.2013.01.063.
This study aims to examine the influence of a 0.5 T static magnetic field (SMF) on yeast and human embryonic kidney (HEK) 293 cell using electrorotation (ROT). Following 48 h exposition to the SMF, no difference was noted between ROT spectra of unexposed and exposed yeast cells, which extend previous reports on the absence of SMF effects on yeast. We also compared the ROT spectrum and the extracted electrical characteristics of HEK cells exposed during 72 h to a 0.5 T uniform static magnetic field with those of unexposed cells. SMF potential effects on HEK proliferation kinetics and cell morphology were also assessed by using the trypan blue exclusion method and scanning electron microscopy, respectively. At last, no significant differences were observed between control and exposed HEK cells concerning electrical properties, growth, and morphology.
本研究旨在使用电动旋转（ROT）技术研究 0.5T 静磁场（SMF）对酵母和人胚肾（HEK）293 细胞的影响。暴露于 SMF 48 小时后，未暴露和暴露酵母细胞的 ROT 光谱没有差异，这延续了先前关于 SMF 对酵母没有影响的报告。我们还比较了在 72 小时内暴露于 0.5T 均匀静磁场的 HEK 细胞的 ROT 光谱和提取的电特性与未暴露细胞的光谱和电特性。此外，我们还使用台盼蓝排斥法和扫描电子显微镜分别评估了 SMF 对 HEK 增殖动力学和细胞形态的潜在影响。最后，在电特性、生长和形态方面，对照和暴露的 HEK 细胞之间没有观察到显著差异。
