Institute of Modern Biopharmaceuticals, School of Pharmaceutical Sciences, Southwest University, Beibei, Chongqing 400716, People's Republic of China.
Biotechnol Lett. 2010 Nov;32(11):1733-7. doi: 10.1007/s10529-010-0375-9. Epub 2010 Aug 10.
We have cloned and analysed a laeA gene (Pci-laeA) that may control mevastatin biosynthesis in Penicillium citrinum. The full-length Pci-laeA sequence is 1,340 bp with an ORF of 1,284 bp encoding 427 amino acids. It shows 95% identity with LaeA from P. chrysogenum. The predicted molecular mass of Pci-LaeA is 48.72 kDa with an estimated theoretical isoelectric point of 6.96. Pci-LaeA has a conserved S-adenosylmethionine binding site and a potential MlcR (a pathway specific regulator in mevastatin biosynthesis) binding site.
我们已经克隆和分析了一个可能控制桔青霉素生物合成的 laeA 基因（Pci-laeA）在青霉中。Pci-laeA 的全长序列为 1340bp，其中包含一个 1284bp 的开放阅读框，编码 427 个氨基酸。它与青霉中的 LaeA 具有 95%的同一性。预测 Pci-LaeA 的分子质量为 48.72kDa，估计理论等电点为 6.96。Pci-LaeA 具有保守的 S-腺苷甲硫氨酸结合位点和潜在的 MlcR（在桔青霉素生物合成中一种特定途径的调节剂）结合位点。
