Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201106, PR China.
J Agric Food Chem. 2010 Sep 8;58(17):9617-21. doi: 10.1021/jf101852e.
Agaricus bisporus fruit bodies were exposed to different doses (1, 2, 3, and 4 kGy) of electron beam irradiation (EBI), and various physiological changes associated with postharvest deterioration, as well as selected enzymes considered to play a role in senescence, were monitored over a subsequent 16-day storage period at 4 degrees C and 75-85% relative humidity. EBI retarded postharvest mushroom softening and overall increases in malondialdehyde levels were more pronounced in controls compared with those of the irradiated samples. After 10 days of storage, polyphenoloxidase activity in samples irradiated with 1-4 kGy doses was significantly (P<0.05) lower compared to that in control samples. Superoxide dismutase activity generally declined throughout the postharvest storage period in both irradiated and control samples, but no clear correlation between enzyme activity and EBI dosage was evident. Catalase activity decreased more slowly and to a lesser extent in fruit bodies exposed to 1 kGy compared with that in the controls and the other irradiated samples.
双孢蘑菇子实体接受不同剂量（1、2、3 和 4 kGy）的电子束辐照（EBI），并在随后的 16 天 4°C 和 75-85%相对湿度下的贮藏期间监测与采后劣变相关的各种生理变化，以及被认为在衰老过程中起作用的选定酶。EBI 延缓了采后蘑菇软化，与辐照样品相比，对照样品中丙二醛水平的整体增加更为明显。贮藏 10 天后，1-4 kGy 剂量辐照样品的多酚氧化酶活性明显（P<0.05）低于对照样品。超氧化物歧化酶活性在辐照和对照样品的整个贮藏期间均普遍下降，但酶活性与 EBI 剂量之间没有明显的相关性。与对照样品和其他辐照样品相比，1 kGy 辐照的子实体中过氧化氢酶活性下降较慢且幅度较小。
