College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, China.
J Agric Food Chem. 2012 Jun 13;60(23):6056-62. doi: 10.1021/jf3006454. Epub 2012 Jun 1.
The influence of methyl jasmonate (MeJA) on postharvest quality and enzyme activities, gene expression level, and the functional component content linked to postharvest deterioration in Agaricus bisporus (J.E. Lange) Imbach fruit bodies was investigated. Freshly harvested fruit bodies were treated with 0 (control), 10 and 100 μM MeJA vapor at 20 °C for 12 h and then stored at 10 °C for up to 7 days. The results indicated that treatments with 100 μM MeJA vapor maintained a high level of soluble protein and total sugar, delayed browning, promoted the accumulation of phenolics and flavonoids, and inhibited the increase of respiratory rate and membrane leakage. Furthermore, 100 μM MeJA inhibited the activities of polyphenoloxidase, increased the antioxidant enzymes activities of catalase and superoxide dismutase, and lowered relative expression levels of three genes encoding polyphenol oxidase (AbPPO1, AbPPO2, and AbPPO3) throughout the storage period. Comparatively, 10 μM MeJA also had a clear beneficial effect on postharvest mushroom quality maintenance but was not as effective as 100 μM MeJA treatment. These findings suggest that application of MeJA could have potential in maintaining the quality of harvested A. bisporus fruit bodies.
研究了茉莉酸甲酯（MeJA）对双孢蘑菇（Agaricus bisporus（J.E. Lange）Imbach）子实体采后品质及与采后劣变相关的酶活性、基因表达水平和功能成分含量的影响。将新鲜采收的子实体在 20°C 下用 0（对照）、10 和 100 μM MeJA 蒸气处理 12 h，然后在 10°C 下贮藏，最长可达 7 天。结果表明，100 μM MeJA 蒸气处理保持了较高水平的可溶性蛋白和总糖，延缓了褐变，促进了酚类和类黄酮的积累，并抑制了呼吸速率和膜渗漏的增加。此外，100 μM MeJA 抑制了多酚氧化酶的活性，增加了过氧化氢酶和超氧化物歧化酶的抗氧化酶活性，并降低了整个贮藏期间编码多酚氧化酶的三个基因（AbPPO1、AbPPO2 和 AbPPO3）的相对表达水平。相比之下，10 μM MeJA 对子实体采后品质的维持也有明显的有益作用，但不如 100 μM MeJA 处理有效。这些发现表明，MeJA 的应用可能有助于维持双孢蘑菇子实体的品质。
