Bidwell J L, Bidwell E A
United Kingdom Transplant Service, Bristol.
Eur J Immunogenet. 1991 Feb-Apr;18(1-2):105-9. doi: 10.1111/j.1744-313x.1991.tb00010.x.
Serological identification of the HLA-DQw1(w5)-associated or HLA-DQw3(w7)-associated DR'Br' (DRB1*0103) allele cannot be accomplished in the presence of a second DQw1(w5)-positive or DQw3(w7)-positive haplotype, respectively. DNA-restriction fragment length polymorphism (RFLP) analysis assists in identification of DR'Br', though not in the presence of DR1. We describe an alternative or complementary method for identification of DR'Br' using two oligonucleotide probes which target HLA-DRB1 gene HV3 regions.
在分别存在第二个DQw1（w5）阳性或DQw3（w7）阳性单倍型的情况下，无法完成与HLA - DQw1（w5）相关或与HLA - DQw3（w7）相关的DR'Br'（DRB1*0103）等位基因的血清学鉴定。DNA限制性片段长度多态性（RFLP）分析有助于DR'Br'的鉴定，不过在存在DR1的情况下则无法鉴定。我们描述了一种使用两个靶向HLA - DRB1基因HV3区域的寡核苷酸探针来鉴定DR'Br'的替代方法或补充方法。
