Combined use of RFLP and PCR-ASO typing for HLA-DR-Dw and DQw typing.
作者信息
Bignon J D, Bidwell J L
机构信息
Laboratoire d'Immunologie Leuco-plaquettaire et d'Histocompatibilité 'France-Transplant', Nantes, France.
出版信息
Eur J Immunogenet. 1991 Feb-Apr;18(1-2):125-38. doi: 10.1111/j.1744-313x.1991.tb00012.x.
Abstract
Due to some limitations of restriction fragment length polymorphism (RFLP) analysis in HLA-DR-DQ typing, we present a combined use of RFLP and polymerase chain reaction (PCR)-allele-specific oligonucleotide (ASO) typing. This scheme consists in selectively amplifying the few RFLP ill-defined genes (DR1/DR'Br' and DR4-Dw subsets) using PCR with allele specific primers to avoid cross-hybridization.
摘要
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