Rezvani Gita, Azarpira Negar, Bita Geramizadeh, Zeynab Rahimi
Department of Oral and Maxillofacial Pathology, Dental School of Shiraz University of Medical Science, Shiraz, Iran.
Indian J Pathol Microbiol. 2010 Jul-Sep;53(3):403-7. doi: 10.4103/0377-4929.68242.
Pyogenic granuloma (PG) is one of the most common reactive vascular lesions in the oral mucosa, which has been divided into the lobular capillary hemangioma (LCH) and the non lobular type (non-LCH) as two distinct entities, on the basis of some investigations.
This study aims to compare the proliferative and angiogenic activity of two histological types of PG to determine whether they have two distinct types of biological behavior.
In this retrospective cross-sectional study, immunostaining was performed on 10 cases of each type of PG.
About 4 microm sections were cut from formalin-fixed paraffin-embedded blocks and each specimen was stained with both anti-CD31 and anti-Ki-67 antibodies simultaneously. Labeling index (LI) was determined for both types by counting Ki-67 and CD31 positive cells separately and simultaneously in 1000 stromal and luminal cells. Micro vessel count (MVC), the mean number of micro vessels in five areas at Chi200 magnification, was also determined for both groups.
The results were statistically compared using the Mann-Whitney U-test.
Ki-67 LI in LCH (5.4 +/- 2.4) was higher than non-LCH (3.9 +/- 3.9). The percentage of CD31 positive cells in LCH (28.5 +/- 22) was lower than non-LCH (37.1 +/- 20.8) and simultaneously immunostaining for both markers in LCH type (2.4 +/- 2.1) was higher than non-LCH (1.2 +/- 1). The MVC was approximately 77.35 +/- 34.6 and 82.6 +/- 42.7 in the lobular areas of LCH and central areas of non-LCH PG, respectively. These differences were not statistically significant.
These results demonstrate a higher proliferation activity in endothelial cells of LCH PG than in non-LCH.
化脓性肉芽肿（PG）是口腔黏膜最常见的反应性血管病变之一，基于一些研究，其已被分为小叶性毛细血管瘤（LCH）和非小叶型（非LCH）两个不同实体。
本研究旨在比较两种组织学类型的PG的增殖和血管生成活性，以确定它们是否具有两种不同类型的生物学行为。
在这项回顾性横断面研究中，对每种类型的PG各10例进行免疫染色。
从福尔马林固定石蜡包埋块上切取约4微米厚的切片，每个标本同时用抗CD31和抗Ki-67抗体染色。通过分别并同时在1000个基质细胞和管腔细胞中计数Ki-67和CD31阳性细胞，确定两种类型的标记指数（LI）。还确定了两组在200倍放大下五个区域微血管计数（MVC），即微血管的平均数量。
使用Mann-Whitney U检验对结果进行统计学比较。
LCH中Ki-67 LI（5.4±2.4）高于非LCH（3.9±3.9）。LCH中CD31阳性细胞百分比（28.5±22）低于非LCH（37.1±20.8），同时LCH型中两种标记物的免疫染色（2.4±2.1）高于非LCH（1.2±1）。LCH小叶区域和非LCH PG中心区域的MVC分别约为77.35±34.6和82.6±42.7。这些差异无统计学意义。
这些结果表明，LCH PG的内皮细胞增殖活性高于非LCH。
