Petrilli P, Sepe C, Pucci P
Istituto di Industrie Agrarie, Università di Napoli, Italy.
Biol Mass Spectrom. 1991 Mar;20(3):115-20. doi: 10.1002/bms.1200200304.
A computer program allowing the correct alignment of peptides generated by a first cleaving agent during protein sequence determination studies has been developed. The program elaborates data obtained from fast atom bombardment mass spectrometric analysis of different digests of the protein. The recorded mass values are used to identify peptides in these digests that overlap peptides from the first cleavage, thus making it possible to establish unambiguously the correct order of these peptides in the protein chain. This procedure has been tested on a model protein by reconstructing the complete sequence of human beta-globin chain, determining the correct alignment of 14 tryptic peptides.
已开发出一种计算机程序，该程序可在蛋白质序列测定研究中实现由第一种裂解剂产生的肽段的正确比对。该程序对从蛋白质不同酶解产物的快原子轰击质谱分析中获得的数据进行详细分析。记录的质量值用于识别这些酶解产物中的肽段，这些肽段与第一次裂解产生的肽段重叠，从而能够明确确定这些肽段在蛋白质链中的正确顺序。通过重建人β-珠蛋白链的完整序列，对14个胰蛋白酶肽段进行正确比对，该程序已在一种模型蛋白质上进行了测试。
