Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, 07745 Jena, Germany.
J Agric Food Chem. 2010 Sep 8;58(17):9418-27. doi: 10.1021/jf1017737.
A liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (HPLC/ESI-TOF-MS) procedure was developed to characterize changes induced in Nicotiana attenuata leaves 1 h and 5 days after wounding and application of Manduca sexta elicitors. The constancy of the measurement conditions was first confirmed for 22 selected analytes spanning the entire chromatogram. Using the Profile Analysis software, we extracted 367 buckets, which were analyzed by principal component analysis and two-factorial ANOVA. One hundred seventy-three buckets were found to be statistically regulated, 128 due to time effects, and 85 due to treatment effects. In vivo 15N-isotope labeling was used to facilitate the annotation and the interpretation of the fragmentation pattern of nitrogen-containing metabolites, and a correlation analysis was performed to test mathematical relationships existing among potential in-source fragments. Additionally, tandem MS measurements of the most regulated ions are presented. Altogether, this study defines a framework for the mining and annotation of major herbivory-elicited changes in Nicotiana attenuata.
建立了一种液相色谱-电喷雾电离-飞行时间质谱（HPLC/ESI-TOF-MS）方法，用于表征受伤后 1 小时和 5 天以及应用烟青虫激发子后，黄花烟草叶片中诱导的变化。首先，通过 Profile Analysis 软件，对横跨整个色谱图的 22 种选定分析物进行了分析，确认了测量条件的稳定性。我们提取了 367 个桶，通过主成分分析和双因素方差分析对其进行了分析。发现 173 个桶具有统计学调节作用，其中 128 个归因于时间效应，85 个归因于处理效应。体内 15N 同位素标记用于促进含氮代谢物的碎片模式的注释和解释，并进行了相关分析以检验潜在源内碎片之间存在的数学关系。此外，还呈现了最受调控离子的串联 MS 测量结果。总的来说，这项研究为挖掘和注释黄花烟草中主要的草食性诱导变化定义了一个框架。
