Department of Orthopedic and Microsurgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
J Neurotrauma. 2010 Oct;27(10):1935-43. doi: 10.1089/neu.2010.1352.
In this study, we aimed to evaluate the potential of tissue-engineered nerve grafts created from acellular allogenic nerve tissues combined with autologous bone marrow stromal cells (BMSCs) for repairing large peripheral nerve lesions. In a rhesus monkey model, a 2.5-cm-long gap was created in the radial nerve, followed by implantation of either autografts or acellular allografts seeded with autologous BMSCs, Schwann cells (SCs), or no cells. Five months after surgery nerve regeneration was assessed functionally, electrophysiologically, and histomorphometrically. Compared to non-cell-laden allografts, BMSC-laden allografts remarkably facilitated the recovery of the grasping functions of the animals. This functional improvement was coupled with increased nerve conduction velocities and peak amplitudes of compound motor action potentials, and greater axon growth, as well as higher target muscle weight. Moreover, the intensities of nerve regeneration in the BMSC-laden group were comparable to those achieved with SC-laden allografts and autografts. Our data highlight the potential of BMSC-seed allografts for the repair of long peripheral nerve lesions, and reveal comparable regeneration intensities achieved by BMSC-, and SC-laden allografts, as well as autografts. Given their wide availability, BMSCs may represent a promising cell source for tissue-engineered nerve grafts.
在这项研究中，我们旨在评估由去细胞同种异体神经组织与自体骨髓基质细胞（BMSCs）结合而成的组织工程神经移植物修复大的周围神经损伤的潜力。在恒河猴模型中，在桡神经上造成 2.5 厘米长的间隙，然后植入自体移植物或种植有自体 BMSCs、施万细胞（SCs）或无细胞的去细胞同种异体移植物。手术后 5 个月，从功能、电生理和组织形态计量学方面评估神经再生。与无细胞负载的同种异体移植物相比，负载 BMSC 的同种异体移植物显著促进了动物抓握功能的恢复。这种功能的改善伴随着神经传导速度和复合运动动作电位峰值幅度的增加，以及更多的轴突生长和目标肌肉重量的增加。此外，BMSC 负载组的神经再生强度与负载 SC 的同种异体移植物和自体移植物相当。我们的数据强调了 BMSC 负载同种异体移植物修复长周围神经损伤的潜力，并揭示了 BMSC 负载、SC 负载同种异体移植物以及自体移植物实现的再生强度相当。鉴于其广泛的可用性，BMSCs 可能是组织工程神经移植物的一种有前途的细胞来源。
