Section of Orthodontics, Department of Oral Growth and Development, Division of Clinical Dentistry, Fukuoka Dental College, Fukuoka, Japan.
Connect Tissue Res. 2011 Feb;52(1):30-5. doi: 10.3109/03008207.2010.502982. Epub 2010 Aug 11.
The elastic system fibers comprise oxytalan, elaunin, and elastic fibers, differing in their relative microfibril and elastin contents. Among them, human periodontal ligament (PDL) contains only oxytalan fibers (pure microfibrils). Elastin microfibril interface-located protein-1 (EMILIN-1) is localized at the interface between microfibrils and elastin. We hypothesized that EMILIN-1 may contribute to the formation of oxytalan fibers. We used a small interfering RNA (siRNA) for EMILIN-1 in PDL cell culture to examine the extracellular deposition of fibrillin-1 (the major component of microfibrils). EMILIN-1 was labeled on microfibrils positive for fibrillin-1 and was colocalized with fibrillin-1 upon immunoprecipitation assay. EMILIN-1 suppression reduced the level of fibrillin-1 deposition to 23% of the control, and this was responsible for the diminution of fibrillin-1 deposition revealed by immunofluorescence. These results suggest that EMILIN-1 may regulate the formation of oxytalan fibers and play a role in their homeostasis.
弹性系统纤维包括羟赖氨酸纤维、弹性蛋白纤维和弹性纤维，它们在相对的微纤维和弹性蛋白含量上有所不同。其中，人牙周韧带（PDL）仅含有羟赖氨酸纤维（纯微纤维）。弹性微纤维界面定位蛋白-1（EMILIN-1）位于微纤维和弹性蛋白之间的界面上。我们假设 EMILIN-1 可能有助于羟赖氨酸纤维的形成。我们在 PDL 细胞培养中使用 EMILIN-1 的小干扰 RNA（siRNA）来检测纤维连接蛋白-1（微纤维的主要成分）的细胞外沉积。EMILIN-1 标记在纤维连接蛋白-1 阳性的微纤维上，并在免疫沉淀测定中与纤维连接蛋白-1 共定位。EMILIN-1 的抑制将纤维连接蛋白-1 的沉积水平降低到对照组的 23%，这是免疫荧光显示纤维连接蛋白-1 沉积减少的原因。这些结果表明，EMILIN-1 可能调节羟赖氨酸纤维的形成，并在其动态平衡中发挥作用。
