基于水溶性 CdTe 量子点探针的小檗碱荧光猝灭法测定
A simple fluorescence quenching method for berberine determination using water-soluble CdTe quantum dots as probes.
机构信息
Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Mar;75(3):1043-6. doi: 10.1016/j.saa.2009.12.050. Epub 2009 Dec 22.
A novel method for the determination of berberine has been developed based on quenching of the fluorescence of thioglycolic acid-capped CdTe quantum dots (TGA-CdTe QDs) by berberine in aqueous solutions. Under optimum conditions, the relative fluorescence intensity was linearly proportional to the concentration of berberine between 2.5x10(-8) and 8.0x10(-6)mol L(-1) with a detection limit of 6.0x10(-9)mol L(-1). The method has been applied to the determination of berberine in real samples, and satisfactory results were obtained. The mechanism of the proposed reaction was also discussed.
已开发出一种基于在水溶液中由小檗碱使巯基乙酸修饰的碲化镉量子点（TGA-CdTe QDs）的荧光猝灭来测定小檗碱的新方法。在最佳条件下，相对荧光强度与小檗碱的浓度在 2.5x10(-8)和 8.0x10(-6)mol L(-1)之间呈线性比例关系，检测限为 6.0x10(-9)mol L(-1)。该方法已应用于实际样品中小檗碱的测定，并获得了满意的结果。还讨论了所提出反应的机制。
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