Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communication, Institute of Photonics Technology, Jinan University, Guangzhou 510632, China.
Clinical Pharmacology Section, Department of Pharmacology, Shantou University Medical College, Shantou 515041, China.
J Am Chem Soc. 2023 May 24;145(20):11311-11322. doi: 10.1021/jacs.3c00243. Epub 2023 Apr 27.
Reliable probing of cardiolipin (CL) content in dynamic cellular milieux presents significant challenges and great opportunities for understanding mitochondria-related diseases, including cancer, neurodegeneration, and diabetes mellitus. In intact respiring cells, selectivity and sensitivity for CL detection are technically demanding due to structural similarities among phospholipids and compartmental secludedness of the inner mitochondrial membrane. Here, we report a novel "turn-on" fluorescent probe for detecting CL in situ. displays outstanding sensitivity and selectivity toward CL through specific noncovalent interactions. In live-cell imaging, its hydrolyzed product efficiently retained itself in intact cells independent of mitochondrial membrane potential (Δψ). The probe robustly co-localizes with mitochondria and outperforms 10--nonyl acridine orange (NAO) and Δψ-dependent dyes with superior photostability and negligible phototoxicity. Our work thus opens up new opportunities for studying mitochondrial biology through efficient and reliable visualization of CL in situ.
在动态的细胞环境中，可靠地探测心磷脂（CL）的含量，这对理解与线粒体相关的疾病（包括癌症、神经退行性疾病和糖尿病），带来了巨大的挑战和机遇。在完整的呼吸细胞中，由于磷脂之间的结构相似性以及线粒体内膜的隔室隔离，CL 的检测在技术上具有选择性和灵敏度的要求。在这里，我们报告了一种用于原位检测 CL 的新型“开启”荧光探针。通过特异性的非共价相互作用， 对 CL 表现出出色的灵敏度和选择性。在活细胞成像中，其水解产物 在不依赖于线粒体膜电位（Δψ）的情况下，能够在完整的细胞内有效地自我保留。该探针与线粒体强烈共定位，其性能优于 10-壬基吖啶橙（NAO）和依赖于 Δψ 的染料，具有更好的光稳定性和可忽略的光毒性。因此，我们的工作为通过高效、可靠地原位可视化 CL 来研究线粒体生物学开辟了新的机会。
