Department of Trauma Surgery, University of Duisburg-Essen, Essen, Germany.
Clin Chem Lab Med. 2010 Nov;48(11):1641-6. doi: 10.1515/CCLM.2010.305. Epub 2010 Aug 13.
Micro-structural changes associated with ultra high molecular weight polyethylene particle (UHMWPE) induced osteolysis, the most frequent cause of aseptic loosening, have been intensively investigated in the mammalian calvarian model by histomorphometry and micro-computed tomography. However, little is known regarding the serological changes that occur during this process.
Serological parameters for bone metabolism [calcium, phosphate, osteocalcin (OCN), deoxypyridinoline (DPD)/creatinine, alkaline phosphatase, osteoprotegerin and receptor activator of nuclear factor-κB] were analyzed in this animal model for particle induced osteolysis. Ten C57BL/6 mice were divided at random into sham operated and UHM-WPE implanted groups. Blood and urine samples were collected prior to and at 14 days after surgery.
Implantation of UHMWPE lead to a significant decrease in bone volume (p=0.027). Both groups (sham/UHMWPE) showed a significant increase in calcium (p=0.004/p=0.027) and phosphate (p=0.001/p=0.001), without correlation to particle implantation. Significantly higher concentrations of DPD/creatinine (p=0.034) and OCN (p=0.022) were found after implantation of UHM-WPE. In addition, parameters could not be correlated to particle induced osteolysis.
DPD can be regarded as a valuable parameter for detecting UHMWPE induced osteolysis in the calvarian model. Further studies of serum parameters should focus on the clinical relevance in aseptic prosthetic loosening.
与超高分子量聚乙烯颗粒（UHMWPE）诱导的骨溶解相关的微结构变化是无菌性松动最常见的原因，在哺乳动物颅骨模型中通过组织形态计量学和微计算机断层扫描进行了深入研究。然而，对于在此过程中发生的血清学变化知之甚少。
在该动物模型中分析了骨代谢的血清学参数[钙、磷、骨钙素（OCN）、脱氧吡啶啉（DPD）/肌酐、碱性磷酸酶、骨保护素和核因子-κB 受体激活剂]，以研究颗粒诱导的骨溶解。将 10 只 C57BL/6 小鼠随机分为假手术组和 UHMWPE 植入组。在手术前和手术后 14 天采集血液和尿液样本。
植入 UHMWPE 导致骨体积明显减少（p=0.027）。两组（假手术/UHMWPE）的钙（p=0.004/p=0.027）和磷（p=0.001/p=0.001）均显著增加，与颗粒植入无关。植入 UHMWPE 后，发现 DPD/肌酐（p=0.034）和 OCN（p=0.022）的浓度显著升高。此外，这些参数不能与颗粒诱导的骨溶解相关。
DPD 可作为检测颅骨模型中 UHMWPE 诱导骨溶解的有价值参数。对血清参数的进一步研究应侧重于无菌性假体松动的临床相关性。
