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一份经过充分特征描述的 MALDI MS 图谱峰列表,用于人类血清。

A well-characterised peak identification list of MALDI MS profile peaks for human blood serum.

机构信息

The BioCentre and Department of Chemistry, University of Reading, Reading, UK.

出版信息

Proteomics. 2010 Sep;10(18):3388-92. doi: 10.1002/pmic.201000100.

Abstract

MALDI MS profiling, using easily available body fluids such as blood serum, has attracted considerable interest for its potential in clinical applications. Despite the numerous reports on MALDI MS profiling of human serum, there is only scarce information on the identity of the species making up these profiles, particularly in the mass range of larger peptides. Here, we provide a list of more than 90 entries of MALDI MS profile peak identities up to 10 kDa obtained from human blood serum. Various modifications such as phosphorylation were detected among the peptide identifications. The overlap with the few other MALDI MS peak lists published so far was found to be limited and hence our list significantly extends the number of identified peaks commonly found in MALDI MS profiling of human blood serum.

摘要

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)分析,使用易于获得的体液(如血清),因其在临床应用中的潜力而引起了极大的关注。尽管已经有许多关于人血清 MALDI-TOF MS 分析的报道,但关于构成这些图谱的物质种类的信息却很少,特别是在较大肽的质量范围内。在这里,我们提供了一个来自人血清的 MALDI MS 图谱峰身份列表,其中包含超过 90 个条目,分子量高达 10 kDa。在肽鉴定中检测到各种修饰,如磷酸化。与迄今为止发表的少数其他 MALDI MS 峰列表的重叠发现是有限的,因此我们的列表大大扩展了通常在 MALDI MS 分析人血清中发现的鉴定峰的数量。

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