Department of Orthopedic Surgery, Tangdu Hospital, The Fourth Military Medical University, Xi' an, China.
BMC Neurosci. 2010 Aug 13;11:98. doi: 10.1186/1471-2202-11-98.
Hydroxysafflor Yellow A (HSYA), which is one of the most important active ingredients of the Chinese herb Carthamus tinctorius L, is widely used in the treatment of cerebrovascular and cardiovascular diseases. However, the potential protective effect of HSYA in spinal cord ischemia/reperfusion (I/R) injury is still unknown.
Thirty-nine rabbits were randomly divided into three groups: sham group, I/R group and HSYA group. All animals were sacrificed after neurological evaluation with modified Tarlov criteria at the 48th hour after reperfusion, and the spinal cord segments (L4-6) were harvested for histopathological examination, biochemical analysis and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining.
Neurological outcomes in HSYA group were slightly improved compared with those in I/R group. Histopathological analysis revealed that HSYA treatment attenuated I/R induced necrosis in spinal cords. Similarly, alleviated oxidative stress was indicated by decreased malondialdehyde (MDA) level and increased superoxide dismutase (SOD) activity after HSYA treatment. Moreover, as seen from TUNEL results, HSYA also protected neurons from I/R-induced apoptosis in rabbits.
These findings suggest that HSYA may protect spinal cords from I/R injury by alleviating oxidative stress and reducing neuronal apoptosis in rabbits.
羟基红花黄色素 A（HSYA）是红花的主要活性成分之一，广泛用于治疗心脑血管疾病。然而，HSYA 对脊髓缺血/再灌注（I/R）损伤的潜在保护作用尚不清楚。
39 只兔子随机分为三组：假手术组、I/R 组和 HSYA 组。所有动物在再灌注后 48 小时根据改良 Tarlov 标准进行神经学评估后处死，取脊髓节段（L4-6）进行组织病理学检查、生化分析和末端脱氧核苷酸转移酶介导的 dUTP-生物素缺口末端标记（TUNEL）染色。
与 I/R 组相比，HSYA 组的神经功能结局略有改善。组织病理学分析表明，HSYA 治疗减轻了 I/R 引起的脊髓坏死。同样，HSYA 治疗后丙二醛（MDA）水平降低，超氧化物歧化酶（SOD）活性增加，表明氧化应激减轻。此外，从 TUNEL 结果可以看出，HSYA 还可以保护兔神经元免受 I/R 诱导的凋亡。
这些发现表明，HSYA 可能通过减轻氧化应激和减少神经元凋亡来保护兔脊髓免受 I/R 损伤。
