Jerzmanowska M, Lorenc R
Acta Physiol Pol. 1978 Mar-Apr;29(2):153-9.
The effects of vitamin D3 on rat kideney metabolism under conditions of experimental hypercalcemia. Acta Physiol. Pol., 1978, 29 (2) 153--159. The effect of vitamin D3 on renal gluconeogenesis processes was studied in the rat. The performed estimations of gluconeogenesis rate from malate and lactate demonstrated significant increases of glucose formation rate when kidney cortex slices of the vitamin D3 treated animals were analyzed. Further studies on the mechanism of the observed phenomenon were performed using kidney cell fractionation procedure and fluorometric estimation of the concentration of selected gluconeogenetic metabolites. Significant increases of phosphoenolpyruvate concentration in the cytosol fraction and citrate concentration in the mitochondrial fraction were observed. Comparison of the described action of vitamin D3 on gluconeogenesis and the effect of parathyriod hormone, as known from the literature suggests similar mechanism of both factor actions. A possibility of vitamin D3 action through the increase in intracellular calcium has been discussed.
实验性高钙血症条件下维生素D3对大鼠肾脏代谢的影响。《波兰生理学学报》，1978年，第29卷（第2期），153 - 159页。研究了维生素D3对大鼠肾脏糖异生过程的影响。对由苹果酸和乳酸生成葡萄糖的速率进行的评估表明，当分析用维生素D3处理的动物的肾皮质切片时，葡萄糖生成速率显著增加。使用肾细胞分级分离程序和荧光法估计所选糖异生代谢物的浓度，对观察到的现象的机制进行了进一步研究。观察到胞质部分磷酸烯醇丙酮酸浓度和线粒体部分柠檬酸浓度显著增加。将维生素D3对糖异生的上述作用与文献中已知的甲状旁腺激素的作用进行比较，表明这两种因子的作用机制相似。讨论了维生素D3通过增加细胞内钙起作用的可能性。
