Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
Urology. 2010 Oct;76(4):1017.e1-6. doi: 10.1016/j.urology.2010.05.045. Epub 2010 Aug 12.
To investigate the expression of Hsp60 protein in prostate cancer biopsy samples, and its association with prognostic clinical parameters and hormone resistance and survival. Molecular chaperones are involved in protein folding, protein degradation, and protein trafficking among subcellular compartments.
We selected 107 patients with localized and locally advanced prostate cancer at our hospital from 1999 through 2004. We performed an analysis by western blot and immunohistochemistry on paraffin-embedded tissue sections. Clinical data were used to determine associations between immunohistochemical expression of Hsp60 and tumor behavior.
The expression level of Hsp60 was significantly increased in tumors with high Gleason score (P < .001). Hsp60 expression was also significantly associated with initial serum PSA levels (P < .01) and with the presence of lymph node metastasis (P < .003). In 50 locally advanced cancers treated by androgen ablation we found an association between high Hsp60-expressing tumors and an early onset of hormone refractory disease (P < .02) and reduced cancer-specific survival (P < .05).
Hsp60 protein is overexpressed in poorly differentiated prostate cancers. Hsp60 expression is strongly associated with prognostic clinical parameters, such as Gleason score, initial serum PSA levels, and lymph node metastasis and with the onset of hormone-refractory disease and reduced cancer-specific survival. Identification of such markers could be of relevance in the clinical management of prostate cancer.
研究热休克蛋白 60（Hsp60）在前列腺癌活检组织中的表达，及其与预后临床参数、激素抵抗和生存的关系。分子伴侣参与蛋白质折叠、蛋白质降解和亚细胞区室之间的蛋白质运输。
我们选择了 1999 年至 2004 年在我院就诊的 107 例局限性和局部进展性前列腺癌患者。我们对石蜡包埋组织切片进行了 Western blot 和免疫组织化学分析。临床数据用于确定 Hsp60 免疫组织化学表达与肿瘤行为之间的关系。
Hsp60 的表达水平在高 Gleason 评分的肿瘤中显著增加（P<0.001）。Hsp60 的表达也与初始血清前列腺特异性抗原（PSA）水平（P<0.01）和淋巴结转移的存在（P<0.003）显著相关。在 50 例接受雄激素剥夺治疗的局部进展性癌症中，我们发现高 Hsp60 表达肿瘤与激素抵抗性疾病的早期发生（P<0.02）和癌症特异性生存时间降低（P<0.05）之间存在关联。
Hsp60 蛋白在分化不良的前列腺癌中过度表达。Hsp60 的表达与预后临床参数密切相关，如 Gleason 评分、初始血清 PSA 水平和淋巴结转移，以及激素抵抗性疾病的发生和癌症特异性生存时间的降低。鉴定这些标志物可能对前列腺癌的临床管理具有重要意义。
