Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Germany.
Biomaterials. 2010 Nov;31(33):8802-9. doi: 10.1016/j.biomaterials.2010.07.071. Epub 2010 Aug 12.
Biomolecule attachment and lateral micropatterning of biomolecular layers are essential techniques to provide in advanced biochemical and cell culture assays. For that purpose, we introduced a versatile, simple and robust method to functionalise standard polystyrene well plates. Free amino groups were generated on the polystyrene surface by low pressure ammonia plasma treatment. Subsequently, thin films of different maleic anhydride copolymers were covalently attached to the surfaces. The distinct physicochemical properties of the coupled maleic anhydride copolymers provided a broad range of possible attachment schemes of proteins and polysaccharides. Micrometer-sized lateral patterns of these functional coatings were created by plasma etching through silicon masks and subsequent chemical conversion of the etched areas using poly(ethylene glycol). The approach facilitates a wide variety of cell culture experiments allowing a combination of biomolecule coupling and micropatterning within the multi-well plate technology.
生物分子附着和生物分子层的横向微图案化是提供先进生化和细胞培养分析的基本技术。为此，我们引入了一种通用、简单和强大的方法来对标准聚苯乙烯微孔板进行功能化。低压氨等离子体处理在聚苯乙烯表面生成游离氨基。随后，将不同的马来酸酐共聚物薄膜共价连接到表面上。偶联的马来酸酐共聚物的独特物理化学性质提供了一系列可能的蛋白质和多糖附着方案。通过等离子体刻蚀通过硅掩模并使用聚（乙二醇）对刻蚀区域进行后续化学转化，在这些功能涂层上形成了微米级的横向图案。该方法促进了各种细胞培养实验，允许在多孔板技术中结合生物分子偶联和微图案化。
