Pompe Tilo, Zschoche Stefan, Herold Nicole, Salchert Katrin, Gouzy Marie-Francoise, Sperling Claudia, Werner Carsten
Institute of Polymer Research Dresden and The Max Bergmann Center of Biomaterials Dresden, Hohe Str. 6, 01069 Dresden, Germany.
Biomacromolecules. 2003 Jul-Aug;4(4):1072-9. doi: 10.1021/bm034071c.
A platform of thin polymer coatings was introduced for the functional modulation of immobilized bioactive molecules at solid/liquid interfaces. The approach is based on covalently attached alternating maleic acid anhydride copolymers with a variety of comonomers and extended through conversion of the anhydride moieties by hydrolysis, reaction with functional amines, and other conversions of the anhydride moieties. We demonstrate that these options permit control of the physicochemical constraints for bioactive molecules immobilized at interfaces to influence important performance characteristics of biofunctionalized materials for medical devices and molecular diagnostics. Examples concern the impact of the substrate-anchorage of fibronectin on the formation of cell-matrix adhesions, the orientation of endothelial cells according to lateral anti-adhesive micropatterns using grafted poly(ethylene oxide), and the spacer-dependent activity of immobilized synthetic thrombin inhibitors.
引入了一个薄聚合物涂层平台，用于在固/液界面处对固定化生物活性分子进行功能调节。该方法基于将交替的马来酸酐共聚物与多种共聚单体共价连接，并通过酸酐部分的水解、与功能性胺的反应以及酸酐部分的其他转化来扩展。我们证明，这些选择允许控制固定在界面处的生物活性分子的物理化学限制，以影响用于医疗设备和分子诊断的生物功能化材料的重要性能特征。实例包括纤连蛋白在底物上的锚定对细胞-基质粘附形成的影响、使用接枝聚环氧乙烷根据横向抗粘附微图案对内皮细胞的定向作用，以及固定化合成凝血酶抑制剂的间隔物依赖性活性。
