Veterinary Public Health Public Laboratory of Guangdong, Veterinary Medicine Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Vet Microbiol. 2011 Jan 27;147(3-4):403-9. doi: 10.1016/j.vetmic.2010.07.012. Epub 2010 Jul 22.
Influenza A viruses of subtype H1N1 have been reported widely in pigs in China, associated with clinical disease. These mainly include classical swine H1N1, avian-like H1N1, and human-like H1N1 viruses. In this study, we reported a novel triple-reassortant H1N1 virus (A/swine/Guangdong/1/2010) containing genes from the classical swine (NP, NS), human (PB1) and avian (HA, NA, M, PB2, PA) lineages, which was for the first time reported in China. Also, phylogenetic analysis further confirmed that five genes segments (NS, NP, PB2, PB1, PA) of the isolate were closely related to the novel reassortant H1N2 viruses isolated in China in 2006, while the other three (HA, NA, M) were closely related to avian-like H1N1 viruses in China. The isolation of triple-reassortant H1N1 influenza virus provides further evidence that pigs serve as emergence hosts or "mixing vessels", and swine influenza virus (SIV) surveillance in China should be given a high priority.
在中国，与临床疾病相关的亚型为 H1N1 的甲型流感病毒已在猪群中广泛报告。这些病毒主要包括经典猪源 H1N1、禽源样 H1N1 和人源样 H1N1 病毒。本研究报道了一种新型的三重重配 H1N1 病毒（A/swine/Guangdong/1/2010），其包含来自经典猪源（NP、NS）、人源（PB1）和禽源（HA、NA、M、PB2、PA）谱系的基因，这是中国首次报告的此类病毒。此外，系统进化分析进一步证实，该分离株的五个基因片段（NS、NP、PB2、PB1、PA）与 2006 年在中国分离的新型重配 H1N2 病毒密切相关，而另外三个（HA、NA、M）与中国的禽源样 H1N1 病毒密切相关。三重重配 H1N1 流感病毒的分离为猪作为新发宿主或“混合容器”提供了进一步的证据，中国应高度重视猪流感病毒（SIV）的监测。
