Dairy Science Department, National Research Center, Dokki, Cairo, Egypt.
Nutrition. 2011 May;27(5):582-9. doi: 10.1016/j.nut.2010.04.002. Epub 2010 Aug 13.
The aims of the present study were to evaluate the antioxidant, radical scavenging, and metal-chelating activity of whey protein concentrate (WPC) and Spirulina alone or in combination in vitro and to evaluate their hepatoprotective effects against CCl(4) in vivo.
Five concentrations (20, 40, 60, 80, and 100 mg/100 mL) of WPC, Spirulina, and their combination were tested in vitro. In the in vivo study, eight groups of male rats comprised the control group and the groups treated with WPC, Spirulina alone, or in combination with or without CCl(4) were used.
The in vitro study showed that WPC and Spirulina showed antioxidant, radical scavenging, and metal-chelating activities in dose-dependent manner. The in vivo study showed that both agents succeeded in preventing liver damage induced by CCl(4). This prevention was more pronounced in rats receiving the combination of WPC and Spirulina.
Whey protein concentrate and Spirulina have free radical scavenging properties and antioxidant activity.
本研究旨在评估乳清蛋白浓缩物（WPC）和螺旋藻单独或联合的体外抗氧化、清除自由基和金属螯合活性，并评估其对 CCl4 的体内肝保护作用。
在体外研究中，测试了 WPC、螺旋藻的五个浓度（20、40、60、80 和 100mg/100mL）及其组合。在体内研究中，使用了八组雄性大鼠，包括对照组和用 WPC、螺旋藻单独或联合治疗的组，以及用或不用 CCl4 治疗的组。
体外研究表明，WPC 和螺旋藻表现出剂量依赖性的抗氧化、清除自由基和金属螯合活性。体内研究表明，两种药物均成功预防了 CCl4 引起的肝损伤。在接受 WPC 和螺旋藻联合治疗的大鼠中，这种预防作用更为明显。
乳清蛋白浓缩物和螺旋藻具有清除自由基和抗氧化活性。
