Department of Immunology, University College London Medical School, Royal Free Hospital Campus, London NW3 2PF, UK.
Vaccine. 2010 Oct 4;28(42):6915-22. doi: 10.1016/j.vaccine.2010.08.004. Epub 2010 Aug 12.
Polysaccharide conjugate vaccines prime for lasting memory responses in children and young adults. The potential value of these vaccines in the elderly is unclear.
We compared the frequency of circulating pneumococcal capsular polysaccharide (PPS) specific IgG, IgA and IgM plasma and memory cells by cultured ELISpot and supernatant screening two years after vaccination with the 7-valent pneumococcal conjugate vaccine (7vCRM) and/or the 23-valent pneumococcal polysaccharide vaccine (PPV) in 252 adults aged 50-80 years. Some individuals received a six-month boost with 7vCRM or PPV. PPS specific IgG memory detected two years post-primary vaccination was correlated with published matched serum IgG concentration pre- and up to one year post-primary vaccination.
There was no difference by vaccine schedule in the quantity of plasma or memory cells detected. The concentration of in vitro PPS IgG produced by memory B cells isolated two years post-vaccination correlated with pre-vaccination serum IgG concentration and not with D28 post-vaccination responses regardless of vaccination schedule.
This study shows that circulating memory B cells numbers two years following immunisation with 7vCRM or PPV are best predicted by pre-vaccination serotype specific serum antibody concentration and not early post-vaccination serum antibody responses.
多糖结合疫苗可在儿童和青年中引发持久的记忆应答。这些疫苗在老年人中的潜在价值尚不清楚。
我们比较了 252 名 50-80 岁成年人在接种 7 价肺炎球菌结合疫苗（7vCRM）和/或 23 价肺炎球菌多糖疫苗（PPV）两年后，通过培养 ELISpot 和上清液筛选，循环肺炎球菌荚膜多糖（PPS）特异性 IgG、IgA 和 IgM 血浆和记忆细胞的频率。一些人接受了 7vCRM 或 PPV 的六个月加强针。两年后初次接种时检测到的 PPS 特异性 IgG 记忆与已发表的匹配血清 IgG 浓度进行了比较，包括初次接种前和初次接种后一年。
疫苗接种方案对检测到的血浆或记忆细胞数量没有差异。两年后从接种疫苗中分离出的记忆 B 细胞产生的体外 PPS IgG 浓度与接种前的血清 IgG 浓度相关，而与接种后 D28 的反应无关，无论接种方案如何。
这项研究表明，接种 7vCRM 或 PPV 两年后循环记忆 B 细胞的数量最好由接种前的血清型特异性血清抗体浓度预测，而不是早期接种后的血清抗体反应。
