通过筛选中国荷斯坦奶牛瘤胃宏基因组文库鉴定的新型糖苷水解酶。
Novel glycoside hydrolases identified by screening a Chinese Holstein dairy cow rumen-derived metagenome library.
机构信息
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
出版信息
Appl Environ Microbiol. 2010 Oct;76(19):6701-5. doi: 10.1128/AEM.00361-10. Epub 2010 Aug 13.
Abstract
One clone encoding glycoside hydrolases was identified through functional screening of a rumen bacterial artificial chromosome (BAC) library. Of the 68 open reading frames (ORFs) predicted, one ORF encodes a novel endo-β-1,4-xylanase with two catalytic domains of family GH43 and two cellulose-binding modules (CBMs) of family IV. Partial characterization showed that this endo-xylanase has a greater specific activity than a number of other xylanases over a wide temperature range at neutral pH and could be useful in some industrial applications.
摘要
通过对瘤胃细菌人工染色体 (BAC) 文库的功能筛选，鉴定出一个编码糖苷水解酶的克隆。在预测的 68 个开放阅读框 (ORF) 中，一个 ORF 编码一种新型内切-β-1,4-木聚糖酶，具有 GH43 家族的两个催化结构域和 IV 家族的两个纤维素结合模块 (CBM)。部分特性表明，该内切木聚糖酶在中性 pH 下的较宽温度范围内比许多其他木聚糖酶具有更高的比活性，并且在某些工业应用中可能有用。
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