Biological College, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha, Hunan 410082, China.
Lab Chip. 2010 Nov 7;10(21):2844-7. doi: 10.1039/c005258b. Epub 2010 Aug 17.
Quantum dots (QDs) are promising fluorescent tags for microarrays. Because most microarrays are analyzed under dry conditions, it is necessary to examine the photo properties of QDs in air. We demonstrate that the photophysical characteristics of individual quantum dots are different at the liquid/solid interface compared with QDs at the air/solid interface by observing them through a wide-field fluorescence microscope. QDs in air show higher photo-stability, higher fluorescence signal, slower spectral blue shift rate, less blinking and shorter bulk fluorescence lifetime than those in solution. These beneficial properties indicate QDs are good alternative fluorescent probes for microarrays.
量子点 (QDs) 是微阵列中很有前途的荧光标记物。由于大多数微阵列都是在干燥条件下进行分析的，因此有必要研究 QD 在空气中的光物理性质。通过宽场荧光显微镜观察，我们发现与在空气/固体界面的 QD 相比，在液体/固体界面的单个 QD 的光物理特性有所不同。与溶液中的 QD 相比，空气中的 QD 具有更高的光稳定性、更高的荧光信号、更慢的光谱蓝移速率、更少的闪烁和更短的体荧光寿命。这些有益的性质表明 QD 是微阵列的一种很好的替代荧光探针。
