Zhou Yingchun, Qian Rongjun, Rao Jing, Weng Mixia, Yi Xuxia
Dpeartment of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2010 Aug;30(4):482-5. doi: 10.1007/s11596-010-0453-1. Epub 2010 Aug 17.
The expression of paired immunoglobulin-like receptor B (PirB) in normal and injured spinal cord of rats was investigated. The SD rat hemi-sectioned spinal cord injury (SCI) model was established. Before and 1, 3, 7, 10 days after SCI, the spinal cord tissues were harvested, and Western blot and immunohistochemistry were used to examine the expression and location of PirB. The results showed that the expression level of PirB in the normal spinal cord of SD rats was low. At the first day after SCI, the expression of PirB was obviously increased, and that in the injured spinal cord from the first day to the 10th day was significantly higher than in the normal spinal cord. The positive expression of PirB in neurons from different regions of gray matter of the injured spinal cord was seen. It was concluded that the expression of PirB in the normal spinal cord of rats was low. The expression of PirB in SCI was significantly increased till at least the 10th day.
研究了配对免疫球蛋白样受体B（PirB）在大鼠正常和损伤脊髓中的表达。建立了SD大鼠半横断脊髓损伤（SCI）模型。在SCI前及SCI后1、3、7、10天，采集脊髓组织，采用蛋白质免疫印迹法和免疫组织化学法检测PirB的表达及定位。结果显示，SD大鼠正常脊髓中PirB的表达水平较低。SCI后第1天，PirB的表达明显增加，且损伤脊髓从第1天至第10天的表达显著高于正常脊髓。可见PirB在损伤脊髓灰质不同区域的神经元中有阳性表达。结论：大鼠正常脊髓中PirB的表达较低。SCI中PirB的表达至少在第10天前显著增加。
