Department of Environmental and Occupational Health, University of Pittsburgh Graduate School of Public Health, Pittsburgh, Pennsylvania, USA.
Ann N Y Acad Sci. 2010 Aug;1203:73-8. doi: 10.1111/j.1749-6632.2010.05558.x.
We have shown that zinc-thiolate moieties of the metal binding protein metallothionein (MT) are critical targets for nitric oxide (NO) with resultant increases in intracellular labile zinc. Such an NO-MT-Zn signaling pathway appears to participate in important cardiovascular functions associated with biosynthesis of NO including hypoxic vasoconstriction in the lung. Although downstream effector signaling molecules and critical contractile targets remain unclear, current investigations reveal a contributory role for zinc dependent protein kinases and cytoskeletal proteins in mediating hypoxic induced constriction of pulmonary endothelial cells.
我们已经表明，金属结合蛋白金属硫蛋白（MT）中的锌巯基部分是一氧化氮（NO）的关键靶标，导致细胞内可移动锌的增加。这种 NO-MT-Zn 信号通路似乎参与了与 NO 生物合成相关的重要心血管功能，包括肺部缺氧性血管收缩。尽管下游效应信号分子和关键收缩靶标仍不清楚，但目前的研究表明，锌依赖性蛋白激酶和细胞骨架蛋白在介导肺内皮细胞缺氧诱导的收缩中起重要作用。
