Medical School, Department of Microbiology and Clinical Microbiology, Ondokuz Mayis University, 55139, Samsun, Turkey.
Curr Microbiol. 2011 Feb;62(2):508-11. doi: 10.1007/s00284-010-9736-9. Epub 2010 Aug 18.
In this study, the effects of 1-(1-naphtylmethyl)-piperazine (NMP), an efflux pump inhibitor, on antimicrobial drug susceptibilities of 42 clinical Acinetobacter baumannii isolates were investigated by the disc diffusion method. The inhibition zone diameters of antibiotic discs were tested in the presence and absence of NMP and then these zone diameters were compared. Presence of NMP restored ciprofloxacin susceptibility in 15 intermediate and 2 resistant isolates. One ciprofloxacin resistant isolate became intermediate in the presence of NMP. One isolate resistant to gentamicin became intermediate with NMP. Interestingly, one isolate susceptible to meropenem became resistant in the presence of NMP. Although NMP increased the inhibition zone diameters of some of the tested antibiotics against the resistant isolates, the increase was not enough to restore susceptibility. In conclusion, the presence of NMP increases the zone diameters of ciprofloxacin and levofloxacin. Intermediate strains become susceptible but the resistant isolates do not.
在这项研究中，通过纸片扩散法研究了外排泵抑制剂 1-（1-萘基甲基）-哌嗪（NMP）对 42 株临床鲍曼不动杆菌分离株的抗菌药物敏感性的影响。在有和没有 NMP 的情况下测试了抗生素纸片的抑菌圈直径，然后比较了这些直径。NMP 的存在使 15 株中介和 2 株耐药的分离株对环丙沙星的敏感性恢复。1 株对环丙沙星耐药的分离株在 NMP 的存在下变为中介。1 株对庆大霉素耐药的分离株在 NMP 的存在下变为中介。有趣的是，1 株对美罗培南敏感的分离株在 NMP 的存在下变为耐药。虽然 NMP 增加了一些耐药分离株对测试抗生素的抑菌圈直径，但增加的幅度不足以恢复敏感性。总之，NMP 的存在增加了环丙沙星和左氧氟沙星的抑菌圈直径。中介菌株变得敏感，但耐药菌株没有。
