Department of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
J Mater Sci Mater Med. 2010 Oct;21(10):2869-79. doi: 10.1007/s10856-010-4135-0. Epub 2010 Aug 18.
Alginate hydrogels in forms of discs and packed beds of microbeads (800 μm) were tested in a novel bioreactor at 10% strain using two regimes: at a loading rate of 337.5 μm/s and at sequential increments of 50 μm displacement every 30 min. Compressive strength increased with the increase in alginate concentration (1.5 vs. 2% w/w) and the content of guluronic residues (38.5 vs. 67%). Packed beds of microbeads exhibited significantly higher (1.5-3.4 fold) compression moduli than the respective discs indicating the effects of gel form and entrapped water. Short-term cultivation of microbeads with immobilized bovine calf chondrocytes (1.5% w/w, 33 × 10(6) cells/ml) under biomimetic conditions (dynamic compression: 1 h on/1 h off, 0.42 Hz, 10% strain) resulted in cell proliferation and bed compaction, so that the compression modulus slightly increased. Thus, the novel bioreactor demonstrated advantages in evaluation of biomaterial properties and cell-biomaterial interactions under in vivo-like settings.
以 10%应变为测试条件，采用两种方式在新型生物反应器中对直径为 800μm 的微球和微球填充床（小球直径 800μm）进行测试：加载速率为 337.5μm/s，每 30 分钟位移增加 50μm。通过增加海藻酸钠浓度（1.5%w/w 对 2%w/w）和古洛糖醛酸残基含量（38.5%w/w 对 67%w/w），压缩强度逐渐增加。微球填充床的压缩模量比相应的微球盘显著提高（1.5-3.4 倍），表明凝胶形式和包埋水的影响。在仿生条件下（动态压缩：1 小时 ON/1 小时 OFF，0.42Hz，10%应变）对固定化牛犊软骨细胞（1.5%w/w，33×10^6 细胞/ml）进行短期培养导致细胞增殖和床层压实，因此压缩模量略有增加。因此，新型生物反应器在评估生物材料性能和细胞-生物材料相互作用方面具有优势，可以模拟体内环境。
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